|Table of Contents|

Changes of Cell Wall Related Enzyme Activities in Different Flesh Peach Fruits During Ripening

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年09
Page:
15-22
Research Field:
Publishing date:

Info

Title:
Changes of Cell Wall Related Enzyme Activities in Different Flesh Peach Fruits During Ripening
Author(s):
GAO Yuanyuan1; CAO Hongbo1; LI Dongdong2; AN Jiale1; ZHANG Xueying1; CHEN Haijiang1
(1.College of Horticulture,Agricultural University of Hebei,Baoding,Hebei 071000；2.Guantao County Agriculture and Rural Bureau,Handan,Hebei 057750)
Keywords:
peach; fleshy fruit; enzyme activity; firmness; respiratory intensity
PACS:
-
DOI:
10.11937/bfyy.20223541
Abstract:
Soft solute type ‘Xiahui 5’,hard solute type ‘Baojiahong’,hard solute type ‘Baojiajun’ and non-solute type ‘Jintong 6’ peach fruit were used as test materials,the activity of enzyme related to cell wall degradation of fruit was determined.The effects of firmness,respiration intensity,cell wall degradation related enzyme activities and gene PG21 and PME expression differences on fruit softening during fruit ripening were studied,in order to clarify the changes of cell wall related enzyme activities during fruit ripening in different fleshy peaches.The results showed that the activities of PME and Cx in ‘Xiahui 5’ peach fruit of soft solute type were higher than those of the other three meat types,and the activities of PG were higher than those of ‘Jintong 6’ and ‘Baojiajun’ peach of hard type,indicated that the activities of PG,PME and Cx were closely related to the softening process of ‘Xiahui 5’ peach fruit of soft solute type.There was no significant difference in the activity of β-gal in peach fruit of four meat types,indicating that β-gal enzyme was not the key factor affecting the peach softening process of different meat types.The relative expression intensity of PG21 gene in four different meat types showed an overall upward trend in the late mature stage of peach fruit,and the relative expression levels of PME gene in ‘Xiahui 5’ and ‘Baojiajun’ showed an upward trend in the whole fruit late development stage,and the change trend of enzyme activity was basically consistent with the expression levels of related genes.

References:

[1]杨本芸.桃、李、杏、核桃不同品种的SSR指纹图谱构建[D].保定:河北农业大学,2006.[2]王倩,郏艳红,孙海波,等.不同耐贮性粉果番茄贮藏期间果实软化相关酶活性的研究[J].保鲜与加工,2020,20(1):72-77.[3]杨勇.不同肉质类型桃贮藏特性和乙烯生物合成及信号转导途径的调控研究[D].南京:南京农业大学,2015.[4]WANG X B,PAN L,WANG Y,et al.PpIAA1 and PpERF4 form a positive feedback loop to regulate peach fruit ripening by integrating auxin and ethylene signals[J].Plant Science,2021,313:111084.[5]曲凤静,韩涛,李丽萍,等.不同浓度乙醛处理对桃果实软化及相关生理代谢的影响[J].中国农业科学,2009,42(1):245-250.[6]茅林春,张上隆.果胶酶和纤维素酶在桃果实成熟和絮败中的作用[J].园艺学报,2001,28(2):107-111.[7]SUNNY G G,SANDY V B,VERLINDEN B E,et al.Pectin modifications and the role of pectin-degrading enzymes during postharvest softening of Jonagold apples[J].Food Chemistry,2014,158:283-291.[8]高华奇,王立芹,孙翠,等.采后跃变型果实软化与果胶降解的研究进展[J].果树学报,2022,39(10):1922-1934.[9]温波,王亚兰,何丽丽,等.桃果胶裂解酶编码基因PpPL1的鉴定及其在果实成熟软化过程中的表达[J].核农学报,2020,34(8):1681-1689.[10]王哲.柑橘酸腐病菌的致病机理及其拮抗酵母的研究[D].武汉:华中农业大学,2012.[11]BRUMMELL D A,LABAVITCH J M.Effect of antisense suppression of endopolygalacturonase activity on polyuronide molecular weight in ripening tomato fruit and in fruit homogenates[J].Plant Physiology,1997,115(2):717-725.[12]陈星星,张斌斌,郭绍雷,等.不同肉质型桃果实成熟过程中乙烯生物合成相关基因的表达差异[J].南京农业大学学报,2020,43(4):637-644.[13]冯新,赖瑞联,高敏霞,等.Adβgal-1和Adβgal-2克隆及其在猕猴桃果实软化中的作用[J].中国农业科学,2019,52(2):312-326.[14]佟兆国,王飞,高志红,等.果胶降解相关酶与果实成熟软化[J].果树学报,2011,28(2):305-312.[15]WHALE S K,SINGH Z,BEHBOUDIAN M H,et al.Fruit quality in ‘Cripp′s Pink’ apple,especially colour,as affected by preharvest sprays of aminoethoxyvinylglycine and ethephon[J].Scientia Horticulturae,2008,115(4):342-351.[16]阎香言,张熠可,李福瑞,等.不同质地桃果实软化过程中细胞壁组分变化的差异[J].北方园艺,2017(20):60-66.[17]WANG D D,YEATS T H,SELMAN U,et al.Fruit softening:Revisiting the role of pectin[J].Trends in Plant Science,2018,23(4):302-310.[18]王仁才,熊兴耀,谭兴和,等.美味猕猴桃果实采后硬度与细胞壁超微结构变化[J].湖南农业大学学报(自然科学版),2000,26(6):457-460.[19]细胞壁多糖降解引起采后菠萝果实成熟软化机理的初步研究[D].湛江:广东海洋大学,2014.[20]BURDON J,MARTIN P,IRELAND H,et al.Transcriptomic analysis reveals differences in fruit maturation between two kiwifruit cultivars[J].Scientia Horticulturae,2021,286:110207.[21]张元薇,辛颖,陈复生.果实软化过程中果胶降解酶及相关基因研究进展[J].保鲜与加工,2019,19(2):147-153.[22]阚娟,刘涛,金昌海,等.硬溶质型桃果实成熟过程中细胞壁多糖降解特性及其相关酶研究[J].食品科学,2011,32(4):268-274.

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Last Update: 2023-06-06