|Table of Contents|

Effects of Exogenous Hormones Pyrimidinol and NAA on the Growth of Regenerated Plants Derived From Garden

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年11
Page:
9-14
Research Field:
Publishing date:

Info

Title:
Effects of Exogenous Hormones Pyrimidinol and NAA on the Growth of Regenerated Plants Derived From Garden
Author(s):
TANG Yongping1; SHENG Wentao2; ZHOU Jinsong1; YIN Yuling1; LUO Shaochun1; CHEN Guangyu1
(1.Institute of Vegetables and Flowers,Jiangxi Academy of Agricultural Sciences,Nanchang,Jiangxi 330200;2.Department of Biological Technology,Nanchang Normal University,Nanchang,Jiangxi 330032)
Keywords:
garden asparagus; ancymidol; NAA; embryoid-derived plant; reproductive efficiency
PACS:
-
DOI:
10.11937/bfyy.20223475
Abstract:
The embryoids obtained from garden asparagus pollen grain culture were used as experimental materials.In the adventitious buds,MS+sucrose 30 g?L-1+agar powder 8 g?L-1 were used as the basic medium,pyrimidinol (0,0.05,0.10,0.50,1.00,2.00 mg?L-1) and naphthylacetic acid (NAA) (0,0.05,0.10,0.20,0.50,1.00 mg?L-1) with different mass concentrations were added,respectively.The effects of pyrimidinol and NAA concentrations on the growth of regenerated plants with different genotypes were studied,in order to optimize and improve the propagation efficiency of garden asparagus (Asparagus officinalis L.) plants in vitro.The results showed that the net growth height of regenerated plants treated with 0.50 mg?L-1 pyrimidinol in the medium increased by 1.05 cm and 1 root respectively,and the root system became longer and the lateral roots increased.After adding 0.50 mg?L-1 pyrimidinol and 0.20 mg?L-1 NAA to MS medium,the net growth height of regenerated plants,the number of stems increased by 1.60 cm and 2.0 roots,respectively,and the root system grew vigorously.And there was no significant difference among the genotypes in the promoting effect of 0.50 mg?L-1 pyrimidinol and 0.20 mg?L-1 NAA on plant regeneration from garden asparagus embryoids.It was speculated that adding 0.50 mg?L-1 pyrimidinol and 0.20 mg?L-1 NAA to MS medium could improve the reproductive efficiency of regenerated plants from garden asparagus embryoids.

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Last Update: 2023-07-25