|Table of Contents|

Soil Bacterial Diversity and Function Prediction Analysis of Poria cocos Cultivating Under Different Planting Modes

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年11
Page:
91-97
Research Field:
Publishing date:

Info

Title:
Soil Bacterial Diversity and Function Prediction Analysis of Poria cocos Cultivating Under Different Planting Modes
Author(s):
XU Lei1; 2; LIU Changli2; GUO Lanping1
(1.National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences，Post-Doctoral Research Station of China Academy of Chinese Medical Sciences,Beijing 100700；2.School of Pharmacy，Hubei University of Chinese Medicine，Wuhan，Hubei 430065)
Keywords:
Poria cocos; high throughput sequencing; bacteria; community structure; diversity
PACS:
-
DOI:
10.11937/bfyy.20223163
Abstract:
Taking the cultivating soil of Poria cocos by different planting modes as experimental materials,using Illumina novaseq high-throughput sequencing technology to study the changes of soil bacterial community before planting and after sclerotium harvest of Poria cocos forest-medicine bionic cultivation and farmland monoculture cultivation.The function of metagenomewas predicted using Tax4fun,in order to provide reference for the suitable cultivation mode and the avoiding continuous cropping mechanism of Poria cocos.The results showed that the number of soil bacterial OTUs increased slightly after two cultivation modes of Poria cocos.Alpha diversity analysis showed that there was no significant difference among Shannon index,Simpson index,Chao1 index and ACE index.The phyla with the highest relative abundance were Proteobacteria,Acidobacteria,Firmicutes,Verrucomicrobia,Crenarchaeota,Actinobacteria,Bacteroidetes,Chloroflexi,Nitrospirae.There was no significant change in the relative abundance of the above-mentioned departments in the farmland monoculture cultivation.In forest-medicine bionic cultivation,the relative abundance of Acidobacteria Verrucomicrobia and Crenarchaeota increased significantly,but did not reach a very significant level.UPGMA cluster analysis showed that the two cultivation patterns were clustered into one category respectively.Tax4fun function prediction was annotated to 6 functions at the first level,the secondary function prediction included 35 sub functions.In summary,the bacterial community structure of Poria cocos cultivated in different models was relatively stable,the number of bacteria increased only a small amount,and the dominant bacterial community did not change significantly.Soil bacteria might not be the main cause of Poria cocos intolerance to continuous cropping.

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Last Update: 2023-07-26