|Table of Contents|

Genetic Diversity Analysis of Morchella Species in China Based on ISSR Marker

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年08
Page:
107-114
Research Field:
Publishing date:

Info

Title:
Genetic Diversity Analysis of Morchella Species in China Based on ISSR Marker
Author(s):
LYU Ruiling; CHEN Shi; WANG Jie; HU Junjun; ZHENG Yongliang
(College of Biology and Agricultural Resources,Huanggang Normal University/Hubei Key Laboratory for the Improvement of Economic Forest Germplasm and Comprehensive Utilization of Resources/Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains,Huanggang,Hubei 438000)
Keywords:
Morchella; ISSR; clustering analysis; polymorphism
PACS:
-
DOI:
10.11937/bfyy.20223042
Abstract:
The inter-simple sequence repeat (ISSR) molecular markers were used to explore the genetic diversity and genetic relationship of natural populations of 49 Morchella samples from 12 province regions．The aim of this study was to reveal the genetic diversity of Morchella resources，and analyze their genetic differences，so as to provide reference for the selection and breeding of Morchella resources．The results showed that the optimal ISSR-PCR reaction system was as follows 10×PCR Buffer (Mg2-) was 2.5 μL,Mg2+ was 20 mmol?L-1,DNA was 1 μg,dNTP Mix was 1 μL,Taq enzyme was 0.4 μL,primer was 1 μL,ddH2O supplemented to 25 μL.and a total of 111 bands were amplified with 5 primers，including 110 polymorphic bands (PPB),The proportion of PPB was 99.10%.The UPGMA method was used for cluster analysis,and 49 samples could be divided into three large groups when the number of different lines was 0.72.In conclusion,there is abundant genetic diversity among cultivated varieties of Morchella in China.

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Last Update: 2023-05-17