|Table of Contents|

Effects of Exogenous GABA on Physiological Indexes of Mycelial of Lentinus edodes Under Different Culture Temperatures

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年04
Page:
111-117
Research Field:
Publishing date:

Info

Title:
Effects of Exogenous GABA on Physiological Indexes of Mycelial of Lentinus edodes Under Different Culture Temperatures
Author(s):
YOU Huafang1; 2; WU Chenyin1; 2; XU Yanjun1; 2; HANG Junnan1; YANG Guo1; XU Juan1; 2
(1.College of Agriculture，Guizhou University，Guiyang，Guizhou 550025；2.Institute of Edible Fungi，Guizhou University，Guiyang,Guizhou 550025)
Keywords:
Lentinus edodes; exogenous GABA; growth inhibition ratio; relief of stress; antioxidant enzymes
PACS:
-
DOI:
10.11937/bfyy.20222381
Abstract:
Lentinus edodes strain LeM-f was used as test material，plates covered with celluloid membrane were cultured at room temperature and subthermal stress to observe the changes of different concentrations of GABA on the growth rate of mycelia，fresh weight of aerogenic mycelia，inhibition rate of mycelia growth，microscopic morphology and antioxidant enzyme activity,in order to provide reference for exploring the effects of GABA on alleviating the stress tolerance and resistance enhancement effect of Lentinus edodes mycelium to subtemperature stress.The results showed that exogenous addition of appropriate concentration of GABA could alleviate the sub-high and low temperature stress suffered by hyphae，promote the growth of mycelium，increase hyphae density，and increase the branching rate of front-end hyphae，and the activity of antioxidant enzymes (CAT，SOD) increased with the addition of exogenous GABA.GABA of 0.6 mmol?L-1 had the best alleviation effect on sub-high stress with a growth inhibition rate as low as 8.63%，which was close to the normal growth level at room temperature，and the yield of aerial mycelium was increased by 212.47% and the branching rate was increased by 34.31% compared with sub-high temperature inhibition，all effects were better than sub-low temperature stress.In summary，exogenous GABA could effectively alleviate the damage caused by sub-high and low temperature，but it had the best effect under sub-high temperature conditions，and the application prospect in production was very wide.

References:

[1]LI L，NA D，HUI Z，et al.The versatile GABA in plants[J].Plant Signaling & Behavior，2021(16):3.[2]RAMOS R R,MARTINEZ F,KNAUF B G.The effects of GABA in plants[J].Cogent Food & Agriculture,2019(5):1670553.[3]AYDIN S，RAHIM N H，MORTEZA S A.Exogenous application of GABA retards cap browning in Agaricus bisporus and its possible mechanism[J].Postharvest Biology and Technology，2021，174:111434.[4]CARILLO P.GABA shunt in durum wheat[J].Frontiers in Plant Science，2018(9):100.[5]WU X，JIA Q，JI S，et al.Gamma-aminobutyric acid (GABA) alleviates salt damage in tomato by modulating Na+uptake，the GAD gene，amino acid synthesis and reactive oxygen species metabolism[J].BMC Plant Biol,2020,20(1):465.[6]LIU C，WANG H，ZHANG X,et al.Activation of the ABA signal pathway mediated by gaba improves the drought resistance of apple seedlings[J].International Journal of Molecular Sciences,2021,22(23):12676.[7]LI Y X，LIU B Y，PENG Y X,et al.Exogenous GABA alleviates alkaline stress in Malus hupehensis by regulating the accumulation of organic acids[J].Scientia Horticulturae,2020,261(9):108982.[8]周莎莎,王刚正,罗义,等.生长素及其类似物增强香菇耐高温性的研究[J].菌物学报,2018,37(12):1723-1730.[9]陆晓晨.根域亚低温胁迫下山定子根系GABA代谢特征及其生理效应研究[D].沈阳:沈阳农业大学,2017.[10]李晓慧,张恩让,何玉安,等.亚高温及外源物质调节下番茄的生理响应[J].江苏农业科学,2013,41(7):135-137.[11]刘秀明.不同食用菌栽培种质对高温胁迫的反应研究[D].北京：中国农业科学院,2017.[12]ORDAZ A,FAVELA E,MENESES M,et al.Hyphal morphology modification in thermal adaptation by the white-rot fungus Fomes sp.EUM1[J].Journal of Basic Microbiology，2012,52(2):167-174.[13]王波,唐利民,熊鹰,等.香菇菌株菌丝和子实体生长耐高温试验研究[J].吉林农业大学学报,2004(2):145-147.[14]刘兵.γ-氨基丁酸和海藻糖对高温胁迫香菇菌丝胞外酶活性的影响[D].太谷:山西农业大学,2017.[15]李玉.烟色离褶伞基内菌丝生长规律及生产中的应用[J].中国食用菌,2009,28(4):18-19.[16]SACHIN K.Complexity of the heat stress response in plants[J].Current Opinion in Plant Biology，2007，10(3)：310-316.[17]郭敬,易美琴,宋波.三种食用菌在温度胁迫下菌丝酶活性的变化研究[J].四川农业科技,2019(5):53-56.[18]李宇星.外源γ-氨基丁酸（GABA）对苹果幼苗碱胁迫的缓解作用及生理机理研究[D].杨凌:西北农林科技大学,2020.[19]李颖,鱼小军,李珍,等.环境胁迫下γ-氨基丁酸对植物生长调控的研究进展[J].草地学报,2022,30(4):835-840.[20]陈凤琼,陈秋森,刘汉林,等.不同外源试剂对菜用大豆低温胁迫的调控效应[J].大豆科学,2022,41(2):165-171.

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Last Update: 2023-03-30