|Table of Contents|

Characterization Analysis of Microsatellite in Chloroplast of Allium mongolicum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年03
Page:
1-7
Research Field:
Publishing date:

Info

Title:
Characterization Analysis of Microsatellite in Chloroplast of Allium mongolicum
Author(s):
WANG Hongxia1; 2; 3; LI Wenqi1
(1.College of Ecological Environment and Resources,Qinghai University for Nationalities,Xining,Qinghai 810000;2.Qinghai Provincial Key Laboratory of High Value Utilization of Characteristic Economic Plants,Xining,Qinghai 810000;3.Laboratory of Biotechnology and Analytical Testing,Xining,Qinghai 810000)
Keywords:
Allium mongolicum; chloroplast; microsatellite
PACS:
-
DOI:
10.11937/bfyy.20222346
Abstract:
Taking Allium mongolicum as test material,the chloroplast genome was sequenced by Illumina NovaSep high-throughput sequencing technology,and the chloroplast genome microsatellites were searched and analyzed by using MISA v1.0 software,in order to provide reference for the species identification,population genetics and phylogenetic studies of A.mongolicum.The results showed that a total of 260 SSR sites were detected on the 153 376 bp chloroplast genome sequence of A.mongolicum.One SSR locus occurred per 590 bp on average,mainly distributed in the large copy region.There were 5 repeat types,of which the largest number of single nucleotide repeat type accounted for 66.79%,followed by trinucleotide (23.99%) and the least number of pentanucleotide,accounting for only 0.37%.The cpSSR was 8-29 bp,mainly in the range of 8-10 bp (75.77%),of which 9 cpSSR were 20 bp and above in length,indicating that the cpSSR on the chloroplast genome of A.mongolicum have a high polymorphic potential.

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Last Update: 2023-02-21