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Isolation and Identification of Endophytes and Actinomycetes From Underwood Planting Ganoderma lucidum

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年03
Page:
120-126
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Endophytes and Actinomycetes From Underwood Planting Ganoderma lucidum
Author(s):
LIU Yueqin; LUO Liyuan; LIU Xinrui; BAI Lu
(College of Life Science,Yan′an University,Yan′an,Shaanxi 716000)
Keywords:
Ganoderma lucidum; endophytes; isolation; identification
PACS:
-
DOI:
10.11937/bfyy.20222032
Abstract:
The underwood planting Ganderma lucidum was used as test material,the effect of different separation methods on the diversity of endophytic bacteria and actinomycetes of G.lucidum was studied by using 16S rDNA identification method,in order to find the strains or new species that produce active ingredients to promote the development of microbial medicine.The results showed that four strains belonging to one genus and one phylum of actinomycetes (LZ-F1,LZ-F2,LZ-F3,LZ-F4) and five strains belonging to four genera and two phyla of bacteria (LZ-B1,LZ-B2,LZ-B3,LZ-B4,LZ-B5) were isolated from healthy G.lucidum fruiting bodies.All four actinomycetes belong to Streptomyces,five strains belong to Peribacillius,Pseudomonas,Neorrhizobium and Bacillus respectively.This study provided technical support for the isolation of other edible fungi,and provided a good reference value for the further development and utilization of active ingredients of G.lucidum.

References:

[1]AHMAD,M F.Ganoderma lucidum：Persuasive biologically active constituents and their health endorsement[J].Biomedicine,Pharmacy,2018,107：507-519.[2]BABY S,JOHNSON A J,GOVINDAN B.Secondary metabolites from Ganoderma[J].Phytochemistry,2015,114：66-101.[3]ZHU J,XU J,JIANG L,et al.Improved antitumor activity of cisplatin combined with Ganoderma lucidum polysaccharides in U14 cervical carcinoma-bearing mice[J].The Kaohsiung Journal of Medical Sciences,2019,35(4)：222-229.[4]LIU Y,LI Y,ZHANG W，et al.Hypoglycemic effect of inulin combined with Ganoderma lucidum polysaccharides in T2dm rats[J].Journal of Functional Foods,2019,55(6)：13-16.[5]CHAN S W,TOMLINSON B,CHAN P,et al.The beneficial effects of Ganoderma lucidum on cardiovascular and metabolic disease risk[J].Pharmaceutical Biology,2021,59(1)：1161-1171.[6]BHARADWAJ S,LEE K E,DWIVEDI V D,et al.Discovery of Ganoderma lucidum triterpenoids as potential inhibitors against Dengue virus NS2B-NS3 protease[J].Scientific Reports,2019,9(1)：1-12.[7]SARGOWO D,OVIANTI N,SUSILOWATI E,et al.The role of polysaccharide peptide of Ganoderma lucidum as a potent antioxidant against atherosclerosis in high risk and stable angina patients[J].Indian Heart Journal,2018,70：608-614.[8]ZHAO C,FAN J,LIU Y,et al.Hepatoprotective activity of Ganoderma lucidum triterpenoids in alcohol-induced liver injury in mice,an iTRAQ-based proteomic analysis[J].Food Chemistry，2019,271:148-156.[9]STEPNIEWSKA Z,KUZNIAR A.Endiphytic microorganisms-promising application in bioremediation of greenhouse grases[J].Applied Microbiology and Biotechnology,2013(97)：9689-9596.[10]MLLER D B,VOGEL C,BAI Y,et al.The plant microbiota：Systems-level insights and perspectives[J].Annual Review of Genetics,2016(50)：211-234.[11]吴锦菲.祁白术内生菌的分离、鉴定及其发酵产物的抑菌活性研究[D].合肥：安徽农业大学,2020.[12]WILSON D.Endophyte：The evolution of a term,and clarification of its use and definition[J].Oikos,1995,73(2)：274-276.[13]曹昆,高福泉,董佳伟，等.人参内生真菌的分离鉴定及其生防功能的初步验证[J].吉林师范大学学报,2021,42(2)：122-126.[14]范冬茹,张德岩,高智涛,等.4种东北食用菌内生菌的分离和纯化[J].中国林副特产,2015(5)：36-38.[15]吴佳,李晓霞,舒伟学,等.缬草内生菌和根际放线菌的分离及安莎类抗生素的筛选[J].西北农林科技大学学报(自然科学版),2019,47(6)：107-114.[16]洪庆华,石璐,孙井梅,等.革兰氏染色三步法应用试验探讨[J].实验室研究与探索,2010,29(11)：15-17.[17]刘朝军,沈定霞.16S rDNA序列测定在细菌鉴定中的应用[J].军医进修学院学报,2011,32(7)：774-776.[18]施宁雪,张烨,倪欢,等.健康草莓体内可分离培养微生物的多样性[J].江苏农业科学,2021,49(7)：130-135.[19]孙红敏,魏玉珍,方晓梅,等.蛇足石杉内生细菌多样性[J].微生物学报,2016,56(4):614-628.[20]WANI Z A,ASHRAF N,MOHIUDDIN T,et al.Plant-endophyte symbiosis,an ecological perspective[J].Applied Microbiology and Biotechnology,2015,99(7)：2955-2965.[21]茵陈内生细菌分离纯化及次级代谢产物活性的研究[D].洛阳:河南科技大学,2020.[22]HALLMANN J,BERG G.Spectrum and population dynamics of bacterial root endophytes[J].Microbial Root Endophytes,2006(5)：15-31.[23]ITO T,MASUBUCHI M.Dereplication of microbial ex-tracts and related analytical technologies[J].The Journal of Antibiotics,2014,67(5)：353-360.[24]何玲敏,叶建仁.植物内生细菌及其生防作用研究进展[J].南京林业大学学报,2014,38(6)：153-159.

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Last Update: 2023-03-07