|Table of Contents|

Optimization of Turbidity Removal Technology for Extracts From Auricularia auricula Polysaccharide by Response Surface Methodology

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年24
Page:
73-89
Research Field:
Publishing date:

Info

Title:
Optimization of Turbidity Removal Technology for Extracts From Auricularia auricula Polysaccharide by Response Surface Methodology
Author(s):
ZHAO Haibing; ZHANG Jian; SONG Guangsen; CHENG Qunpeng; RAN Han; QU Mengjie
(School of Chemical & Environmental Engineering,Wuhan Polytechnic University,Wuhan,Hubei 430023)
Keywords:
Auricularia auricular; polysaccharide; turbidity; activated clay; response surface methodology
PACS:
-
DOI:
10.11937/bfyy.20221845
Abstract:
The effects of acidity of the extract,the amount of activated clay,the treatment temperature and time on the clearance efficiency of turbidity and the recovery of polysaccharide were investigated by using the extract of polysaccharides from Auricularia auricularia and evaluation with turbidimetry and phenol-sulfuric acid methods.Box-Behnken experiment design was employed to establish and analyze the mathematical model of the influence of each factor on the removal efficiency of turbidity and the recovery of crude polysaccharide,in order to provide reference for the rational development and utilization of the extract of crude polysaccharides from Auricularia auricularia.The results showed that the protein content in the extract was reduced from 0.39 mg·mL-1 to 0.05 mg·mL-1 after treatment with activated clay.The optimal process conditions for removal of the turbidity in the extract were as follows,the recovery of polysaccharides and removal efficiency of turbidity were 85.7% and 61.0% respectively by addition of activated clay at a solid-liquid ratio of 1.86 g·mL-1 to the extract (pH 7.0) and treatment at 44.8 ℃ for 61.2 minutes.

References:

[1]GU J,LI Q,LIU J,et al.Ultrasonic-assisted extraction of polysaccharides from Auricularia auricula and effects of its acid hydrolysate on the biological function of Caenorhabditis elegans[J].International Journal of Biological Macromolecules,2021,167:423-433.[2]NGUYEN T L,CHEN J,HU Y,et al.In vitro antiviral activity of sulfated Auricularia auricula polysaccharides[J].Carbohydrate Polymers,2012,90:1254-1258.[3]XU S,ZHANG Y,JIANG K.Antioxidant activity in vitro and in vivo of the polysaccharides from different varieties of Auricularia auricular[J].Food & Function,2016,7(9):3868-3879.[4]NGUYEN T L,WANG D,HU Y,et al.Immuno-enhancing activity of sulfated Auricularia auricula polysaccharides[J].Carbohydrate Polymers,2012,89:1117-1122.[5]暴增海,石桐磊.食用菌的抑菌作用[J].北方园艺,2012(18):174-175.[6]ZHAO Y,YANG Q S,WANG B J,et al.Optimization of decolorization technology of polysaccharides from Lethariella spp.with activated carbon[J].Advanced Materials Research,2014,962/965:1226-1230.[7]SHAO L,SUN Y,LIANG J,et al.Decolorization affects the structural characteristics and antioxidant activity of polysaccharides from Thesium chinense Turcz:Comparison of activated carbon and hydrogen peroxide decolorization[J].International Journal of Biological Macromolecules,2020,155:1084-1091.[8]AM M,AABY K.Optimization of extraction of apple pomace phenolics with water by response surface methodology[J].Journal of Agricultural and Food Chemistry,2010,58(16):9103-9111.[9]WIJNGAARD H,BRUNTON N.The optimization of extraction of antioxidants from apple pomace by pressurized liquids[J].Journal of Agricultural and Food Chemistry,2009,57(22):10625-10631.[10]SHI Y,YUAN Z,XU T,et al.An environmentally friendly deproteinization and decolorization method for polysaccharides of Typha angustifolia based on a metal ion-chelating resin adsorption[J].Industrial Crops and Products,2019,134(2):160-167.[11]KAYNAK G,ERSOZ M,KARA H.Investigation of the properties of oil at the bleaching unit of an oil refinery[J].Journal of Colloid & Interface Science,2004,280(1):131-138.[12]ROSSIi M,GIANAZZA M,ALAAMPRESE C,et al.The role of bleaching clays and synthetic silica in palm oil physical refining[J].Food Chemistry,2003,82(2):291-296.[13]WANG J,JI Z,ZHAO B,et al.A comparison study on microwave-assisted extraction of Potentilla anserina L.polysaccharides with conventional method:Molecule weight and antioxidant activities evaluation[J].Carbohydrate Polymers,2010,80(1):84-93.[14]ZHI Y,HAN X,LI J.Ultrasound-assisted extraction of polysaccharides from mulberry leaves[J].Food Chemistry,2011,127(3):1273-1279.[15]ZENG W C,ZHANG Z,GAO H,et al.Characterization of antioxidant polysaccharides from Auricularia auricular using microwave-assisted extraction[J].Carbohydrate Polymers,2012,89(2):694-700.[16]DUBOIS M,GILLES K A,HAMILTON,et al.Colorimetric method for determination of sugars and related substances[J].Analytical Chemistry,1956,28(3):350-356.[17]MARION M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976(7):248-254.[18]WU F C,TSENG R L,JU R S.Kinetics of color removal by adsorption from water using activated clay[J].Environmental Technology,2001,22(6):721-729.[19]CHEN T C,SAPITANC J,BALLESTEROS F C,et al.Using activated clay for adsorption of sulfone compounds in diesel[J].Journal of Cleaner Production,2016,124:378-382.[20]WENG C H,SHARMA Y C,CHU S H.Adsorption of Cr (VI) from aqueous solutions by spent activated clay[J].Journal of Hazardous Materials,2008,155(1/2):65-75.[21]HEARON S E,WANG M,MCDONALD T J,et al.Decreased bioavailability of aminomethylphosphonic acid (AMPA) in genetically modified corn with activated carbon or calcium montmorillonite clay inclusion in soil[J].Journal of Environmental Sciences,2021,100:131-143.[22]LI X,MA R,HUANG F,et al.Adsorption of naphthenic acids from dewaxed vacuum gas oil by activated clay:Kinetics,equilibrium and thermodynamic studies[J].China Petroleum Processing and Petrochemical Technology,2017,19(1):123-134.[23]CHEN G,LUO Y C,JI B P,et al.Effect of polysaccharide from Auricularia auricula on blood lipid metabolism and lipoprotein lipase activity of ICR mice fed a cholesterol-enriched Diet[J].Journal of Food Science,2008,73(6):103-108.[24]李晶,张洪霞,朱鸿敏,等.星点设计—效应面法优化升阳散火汤提取工艺[J].沈阳药科大学学报,2010,27(5):392-397.

Memo

Memo:
-
Last Update: 2023-01-22