|Table of Contents|

Analysis of the Effects of Pesticides on Microbial Diversity of Lycium chinense L.in Ningxia Based on Macrogenomics

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年01
Page:
98-105
Research Field:
Publishing date:

Info

Title:
Analysis of the Effects of Pesticides on Microbial Diversity of Lycium chinense L.in Ningxia Based on Macrogenomics
Author(s):
LIN Wenxing1; MA Pengsheng1; WANG Fang2; ZHOU Li1; LIU Cheng1; WU Xiuli1
(1.College of Pharmacy，Ningxia Medical University/Key Laboratory of Ningxia Ethnomedicine Modernization，Ministry of Education at Ningxia Medical University，Yinchuan，Ningxia 750004；2.Institute of Plant Protection，Ningxia Academy of Agriculture and Forestry Sciences，Yinchuan,Ningxia 750004)
Keywords:
Lycium chinense L.; pesticides; microorganism; metagenome sequencing
PACS:
-
DOI:
10.11937/bfyy.20221660
Abstract:
Taking the soil samples before and after pesticide spraying in the Lycium fields of three different production areas in Ningxia as the research object,the Illumina sequencing platform to carry out the high throughput sequencing of their macrogenomes to study the impact of pesticides on the soil microbial community in the Lycium fields in Ningxia, in order to provide reference for the ecological environment protection of the Lycium fields and the development and utilization of pesticide degrading bacteria.The results showed that there were 89 phyla，2 102 genera and 8 337 species identified.Pesticides led to the reduction of Shannon index in different areas as well as the soil microbial community diversity of Lycium chinense L..The pesticides increased the relative abundance of the major phyla of the soil in the phylum Aspergillus，Candidatus Tectomicrobia，as well as the major genera Gaiella, Candidatus entotheonella, Nitrosospira, Euzebya and Nitriliruptor.Besides，it also increased the relative abundance of some pathogenic bacteria genera.Functional predictions of soil microorganisms were mainly involved in 45 sub-functions such as global and loverview maps，carbohydrate metabolismand amino acid metabolism，showing functional richness.In short,the use of pesticide reduced the overall metabolic capacity of soil microorganisms.

References:

[1]徐常青,刘赛,徐荣,等.我国枸杞主产区生产现状调研及建议[J].中国中药杂志,2014,39(11):1979-1984.[2]DENNIS P G,KUKULIES T,FORETNER C,et al.The effects of glyphosate，glufosinate，paraquat and paraquat-diquat on soil microbial activity and bacterial，archaeal and nematode diversity[J].Sci Rep,2018(8):2199.[3]XU X H,ZARECKI R,MEDINA S,et al.Modeling microbial communities from atrazine contaminated soils promotes the development of biostimulation solutions[J].ISME J,2019,13(2):494-508.[4]陆涛,李燕,傅正伟,等.农药对根际微生物群落的影响及潜在风险[J].农药学学报,2019,21(Z1):865-870.[5]SINGH R P,JOHRI A K,DUA M.Metagenomic analysis of microbial diversity in cotton rhizosphere soil in Alwar，India[J].Microbiology Resource Announcements,2020,49(9):e00987.[6]DATTA S,RAJNISH K N,SAMUEL M S,et al.Metagenomic applications in microbial diversity，bioremediation，pollution monitoring，enzyme and drug discovery:A review[J].Environmental Chemistry Letters,2020（18）:627569.[7]郝海婷,王若愚,赵霞,等.基于高通量测序技术的堆肥对兰州百合根际微生物多样性的影响[J].西北农业学报,2017,26(3):437-447.[8]程娟,万婷婷,赵慧慧,等.毒死蜱对棉花根际土细菌群落多样性和结构的影响[J].中国生态农业学报,2016,24(5):652-659.[9]FANG H,YU Y L,CHU X Q,et al.Degradation of chlorpyrifos in laboratory soil and its impact on soil microbial functional diversity[J].Journal of Environmental Sciences,2009,21(3):380-386.[10]沙月霞.吡虫啉对枸杞土壤微生物群落功能多样性的影响[J].西北农业学报,2015,24(9):119-126.[11]NETTLES R,WATKINS J,RICKS K,et al.Influence of pesticide seed treatments on rhizosphere fungal and bacterial communities and leaf fungal endophyte communities in maize and soybean[J].Appl Soil Ecol,2016,102:61-69.[12]纳小凡,郑国琦,彭励,等.不同种植年限宁夏枸杞根际微生物多样性变化[J].土壤学报,2016,53(1):241-252.[13]NEWMAN M M,HOILETT N,LORENZ N,et al.Glyphosate effects on soil rhizosphere associated bacterial communities[J].Science of the Total Environment,2016,543(Part A):155-160.[14]高雪峰,韩国栋.短花针茅根系分泌物对荒漠草原土壤细菌群落及土壤养分的影响[J].中国草地学报,2021,43(6):76-84.[15]孙永琦.毛乌素沙地地衣结皮层微生物的群落结构及其固碳功能[D].北京:北京林业大学,2019.[16]向君亮,刘权,申永瑞,等.松嫩草原盐碱土细菌多样性分析[J].干旱地区农业研究,2020,38(2):62-68.[17]PENG M,JIA H B,WANG Q Y.The effect of land use on bacterial communities in saline-alkali soil[J].Current Microbiology,2017,74(3):325-333.[18]JEFFRIES T C,SMRITI R,NIELSEN U N,et al.Metagenomic functional potential predicts degradation rates of a model organophosphorus xenobiotic in pesticide contaminated soils[J].Frontiers in Microbiology,2018(9):147.[19]祁慧鹓,朱国繁,王鑫伟,等.农药污染土壤生物刺激修复技术研究进展[J].土壤,2021,53(2):221-228.[20]王晨.西北荒漠草原土壤硝化菌群结构和活性的研究[D].兰州:兰州大学,2019.[21]李佳佳,李昭煜,漆永红,等.枸杞炭疽病生防菌株筛选、生物功能测定及防治效果[J].植物保护学报,2020,47(6):1343-1352.[22]黄博闻,崔凯,余向阳,等.噻虫嗪胁迫对水稻内生细菌群落结构和多样性的影响[J].江苏农业学报,2021,37(6):1370-1377.[23]靳晓拓,周彦妤,夏杨荣畅,等.多效唑对芒果园土壤细菌多样性的影响及PICRUSt基因功能预测分析[J].热带作物学报,2019,40(4):807-814.

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Last Update: 2023-01-31