|Table of Contents|

Optimization of Extraction Conditions of Flavonoids From Akebia trifoliata by Response Surface Methodology and Its Antioxidant Activity

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年21
Page:
100-105
Research Field:
Publishing date:

Info

Title:
Optimization of Extraction Conditions of Flavonoids From Akebia trifoliata by Response Surface Methodology and Its Antioxidant Activity
Author(s):
SU Huihui; XU Fei; WU Dingkun; DU Jianing; MA Jinshuo
(Sanquan College of Xinxiang Medical University,Xinxiang,Henan 453003)
Keywords:
Akebia trifoliata ; total flavonoids; response surface methodology; antioxidant activity
PACS:
-
DOI:
10.11937/bfyy.20221345
Abstract:
Taking Akebia trifoliata as test material,the extraction technology of total flavonoids from Akebia trifoliata was optimized by single factor test combined with response surface method，in order to provide reference for the further development and utilization of Akebia trifoliata .The results showed that the optimal extraction conditions were 56% ethanol volume fraction,70 minutes extraction time,90 ℃ extraction temperature and 50∶1 mL·g-1 liquid-solid ratio.Under these conditions,the amount of flavonoids extracted was 60.74 mg·g-1,which was basically consistent with the predicted value of 60.492 4 mg·g-1,indicating that the extraction process was stable and feasible.The total flavonoids obtained under the optimal process conditions had a high scavenging ability on 1,1-diphenyl-2-trinitrophenylhydrazine (DPPH) free radical,indicating that the total flavonoids of Akebia trifoliata had a good antioxidant activity.

References:

[1]国家中药学管理局《中华本草》编委会.中华本草[M].上海:上海科学技术出版社,1998.[2]贾明良,方荷芳,李同建,等.三叶木通愈伤诱导及分化研究[J].中国农业科技导报,2020,22(3):181-187.[3]郭林新,马养民,乔珂,等,三叶木通化学成分及其抗氧化活性[J].中成药,2017,39(2):338-342.[4]IWANAGA S,WARASHINA T,MIYASE T.Triterpene saponins fromthe pericarps of Akebia trifoliate [J].Chem Pharm Bull,2012,60(10):1264-1274．[5]万明长,刘学武,班小重,等．三叶木通栽培条件下果实性状及营养成分分析[J].贵州农业科学,2008,36(3):121-122.[6]邵显会,肖娅萍,张珂,等.不同产地三叶木通中总黄酮含量的测定[J].光谱实验室,2012,29(3):1345-1349.[7]张可兰,韦日明,葸博婷,等.三叶木通总黄酮对急性肝损伤小鼠的保护作用及机制研究[J].中药材,2018,41(3):707-710.[8]魏永生,王永宁,石玉平,等.分光光度法测定总黄酮含量的实验条件研究[J].青海大学学报(自然科学版),2003,21(3):61-63.[9]臧青民,李秋珊,徐燕波,等．超声波辅助双水相体系优化橘红花 总黄酮提取工艺及其抗氧化活性[J]．化学试剂,2022,44(4):4-5.[10]WANG Z J,ZHAO X H,ZHAO D M,et al.Ultrasonic microwave-assisted micellar extraction and purification of flavonoids from licorice by metal complex and antisolven trecry stallization[J].LWT-Food Sci Technol,2021,147:111501.[11]桂利利,吴正坤,余惠凡,等.黄药叶总黄酮提取工艺优化及其抗氧化活性研究[J]．食品研究与开发,2021,42(19):100-108.[12]李勇,邓卓丹,许均华.响应面超声提取鳄梨皮果胶工艺及其氧化特性[J].食品工业,2021,42(11):15-20.

Memo

Memo:
-
Last Update: 2023-01-16