|Table of Contents|

Cloning and Expression Analysis of DcZDS Gene in Carrot

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年22
Page:
9-15
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of DcZDS Gene in Carrot
Author(s):
AN Wenjie1; ZHANG Yanan2; CHANG Shuangfeng2; WU Zhe2
(1.Department of Horticulture，Shanxi Forestry Vocational Technical College，Taiyuan，Shanxi 030001；2.College of Horticulture，Shanxi Agricultural University，Taigu，Shanxi 030801)
Keywords:
Daucus carota L; ζ-carotene dehydrogenase; gene cloning; expression analysis
PACS:
-
DOI:
10.11937/bfyy.20221274
Abstract:
Three varieties of carrot ‘Yellow coral’ (19071)，orange carrot ‘New Kuroda Wu’ (C1)，red carrot ‘Rose’ (17106) were used as test materials.Bioinformatics methods were used，the amino acid composition and physicochemical properties of DcZDS gene obtained from three varieties of carrot clones and the relative expression of DcZDS gene in taproot of three varieties of carrot at different developmental stages were studied，in order to provide reference for the effect of DcZDS gene on lycopene synthesis.The results showed that the cDNA full-length of DcZDS gene was 1 722 bp，encoding 573 amino acids.The physical and chemical properties of DcZDS gene encoded proteins were basically the same，all belonged to hydrophilic and unstable proteins.The secondary structures of the encoded proteins mainly contained α-helix，irregular curl and mainly played a role in chloroplasts，these proteins were non-secretory proteins.Phylogenetic tree analysis showed that the DcZDS gene in carrot was the closest relative to DcZDS in celery.The expression of DcZDS gene increased first and then decreased at different stages of taproot development，but the expression of DcZDS in red taproot was significantly higher than that in orange and yellow taproot，65 days after taproot development.The results showed that the high expression of DcZDS promoted the accumulation of lycopene in red carrot.

References:

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Last Update: 2023-01-17