|Table of Contents|

Study on Classification of Oudemansiella raphanipes Based on HPCE and Multi Gene Combined Sequence Identification

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年18
Page:
117-123
Research Field:
Publishing date:

Info

Title:
Study on Classification of Oudemansiella raphanipes Based on HPCE and Multi Gene Combined Sequence Identification
Author(s):
GONG Na; MA Xiaoying; CHEN Xun; LIU Guoli; ZHAO Ying; XIAO Jun
(Institute of Edible Fungi,Liaoning Academy of Agricultural Sciences,Shenyang，Liaoning 110161)
Keywords:
Oudemansiella raphanipes; capillary electrophoresis; multigene analysis
PACS:
-
DOI:
10.11937/bfyy.20221010
Abstract:
Five isolates of Oudemansiella raphanipes were used as the experimental materials.The molecular biological identification of Oudemansiella raphanipes was determined and comprehensive analysised by using ITS and nLSU sequence,combined with the sequences of related species in GenBank and morphological characteristics of the strains. In addition,high performance capillary electrophoresis was used to study the high performance capillary fingerprinting of Oudemansiella raphanipes,and the identification and evaluation were carried out by principal component analysis,similarity analysis and cluster analysis,in order to explore a new rapid and accurate identification method and to solve the problem of confusing nomenclature problems due to the large number of species of Oudemansiella raphanipes.The results showed that all test strains had close relationships,were all Oudemansiella raphanipes.Strains with close genetic relationships of Or2,Or5 and Or1 all clustered into one branch to varying degrees,Or2,Or5 and Or1 had closer genetic relationships than Or4 and Or6.The ‘W’ shaped triad differential peak was used as the key indicator peak for the identification of different fruiting bodies of Oudemansiella raphanipes.There was rich species diversity in Oudemansiella raphanipes.The identification method of HPCE and multi gene joint sequence could effectively distinguish different morphological species,which provided reference for rapid screening of high-quality Oudemansiella raphanipes strains.

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Last Update: 2022-11-30