|Table of Contents|

Optimization of Polyphenols Extraction From Schefflera arboricola Hayata by Response Surface Methodology and Its Antioxidant Activity

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年18
Page:
90-98
Research Field:
Publishing date:

Info

Title:
Optimization of Polyphenols Extraction From Schefflera arboricola Hayata by Response Surface Methodology and Its Antioxidant Activity
Author(s):
YANG Shenming; NING Na; YANG Xiao; MA Jinlin; LI Zhengjiang; WANG Zhenji
(Department of Resources，Environment and Chemistry，Chuxiong Normal University,Chuxiong，Yunnan 675000)
Keywords:
Schefflera arboricola Hayata; polyphenols; ultrasound extraction; response surface methodology; antioxidant activity
PACS:
-
DOI:
10.11937/bfyy.20220725
Abstract:
Taking Schefflera arboricola Hayata as the test material，extraction of polyphenols from Schefflera arboricola Hayata by cellulase combined with ultrasound was studied，the extraction conditions were optimized by univariate and response tests and the scavenging capacity of polyphenols from Schefflera arboricola Hayata on hydroxyl radicals (·OH)，DPPH radicals (DPPH·) and nitrite (NO-2) was also studied,in order to provide reference for the comprehensive development and utilization of Schefflera arboricola Hayata polyphenols.The results showed that the optimum extraction conditions of polyphenols from Schefflera arboricola Hayata were ethanol volume fraction 40%，solid-liquid ratio 1:35 g·mL-1，enzymolysis hydrolysis time 35 minutes，ultrasonic power 350 W，enzymolysis temperature 50 ℃，cellulase dosage 3.0%(with the quality of Schefflera arboricola Hayata，the same below).Under these conditions，the extraction amount of polyphenols from Schefflera arboricola Hayata was (15.52±0.824) mg·g-1，which was close to the predicted value (15.61 mg·g-1).Schefflera arboricola Hayata polyphenols showed strong scavenging effects on hydroxyl radicals，DPPH radicals and nitrite，and increased with the increase of polyphenols concentration.When the concentration of Schefflera arboricola Hayata polyphenols was 0.137 mg·mL-1，the clearance rates of ·OH，DPPH· and NO-2 were 54.33%，91.14% and 61.42% respectively.The extraction process parameters optimized by response method were accurate and reliable，and could be used for the extraction of polyphenols from Schefflera arboricola Hayata.Schefflera arboricola Hayata polyphenols had strong antioxidant capacity in vitro and could be used as natural antioxidant resources in food and medicine.

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Last Update: 2022-11-30