|Table of Contents|

Cloning and Expression Characterization of Heat-resistant Gene [STBX]HSFA2[STBZ] in Wild Lily

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年22
Page:
67-73
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Characterization of Heat-resistant Gene [STBX]HSFA2[STBZ] in Wild Lily
Author(s):
JIANG Yao1; CHEN Wenbo1; CHEN Qibing2
(1.College of Health,Kaili University,Kaili,Guizhou 556011；2.College of Landscape Architecture,Sichuan Agricultural University,Chengdu,Sichuan 611130)
Keywords:
wild lily; [STBX]HSFA2[STBZ] gene; genetic expression; heat-resistance
PACS:
-
DOI:
10.11937/bfyy.20220715
Abstract:
Taking leaves of wild lily as test materials which from Southeast of Guizhou prefecture, the effects on cloning and expression characterization of heat-resistant gene 〖STBX〗HSFA2 〖STBZ〗in wild lily were studied by RACE and qRT-PCR method,in order to provide reference for improving the heat tolerance and resistance improvement of wild lily in Southeast of Guizhou.The results showed that the full length with 1 435 bp cDNA of 〖STBX〗HSFA2 〖STBZ〗gene was isolated 〖STBX〗HSFA2 〖STBZ〗gene,containing with 5′-untranslated region of 77 bp, 3′-untranslated region of 155 bp and open reading frame of 1 203 bp which encoding 400 amino acid with a molecular weight of 45.3 kD.This protein was highly homologous to know HSFA2,which sharing 87.56% and 83.64% identities with HSFA2 from Populus trichocarpa and Populus simonii respectively.The results showed that highly conserved DNA binding from the different species was in this protein.qRT-PCR analysis indicated that the expression leve l of HFA2 in root,bulbs and leaves was obviously different,but which in leaves of wild lily was significantly higher than that in root and bulbs.The relative expression level of 〖STBX〗HSFA2 〖STBZ〗gene was significantly elevated during 3—12 hours after heat treatment,but the expression level was down-regulated at 24 hours,which was higher than wild type.The relative expression levels of OE-5 and OE-7 transgenic lines which reaching the peak,were greater than that of non-transgenic plants.Photochemical quenching coefficient and chlorophyll content of transgenic plant were higher than that in non-transgenic plants after heat treatment.Furthermore,the results showed that the ability of transgene lines to heat stress was similar to that of Arabidopsis.This research obtained the different transgenic lines with higher resistance ability to heat stress,which was a highly conserved up-regulator to heat stress.

References:

［1］易瑾,罗弦,曹兴,等.百合热激转录因子基因LlHSF1的克隆与表达分析［J］.园艺学报,2012,39(11):2199-2205.［2］GUO J,WU J,QIAN J,et al.Genome-wide analysis of heat shock transcription factor families in rice and Arabidopsis［J］.Journal of Genetics and Genomics,2008,35(2):105-118.［3］陈勇兵,王燕,张海利.番茄热激转录因子(Hsf)基因家族鉴定及表达分析［J］.农业生物技术学报,2015,23(4):492-501.［4］LIN Y X,JIANG H Y,CHU Z X,et al.Genome-wide identification,classification and analysis of heat shock transcription factor family in maize［J］.BMC Genomics,2011,12(1):76-89.［5］XUE G P,SADAT S,DRENTH J,et al.The heat shock factor family from Triticum aestivum in response to heat and other major abiotic stresses and their role in regulation of heat shock protein genes［J］.Journal of Experimental Botany,2014,65(2):539-557.［6］张蓓,胡文霞,靳燚,等.白魔芋热激转录因子AaHSFA2a和AaHSFA2c的分离,亚细胞定位及表达分析［J］.分子植物育种,2018,16(24):8005-8012.［7］SCHARF K D,BERBERICH T,EBERSBERGER I,et al.The plant heat stress transcription factor (Hsf) family:Structure,function and evolution［J］.Biochim Biophys Acta Gene Regul.Mech,2012,1819(2):104-119.［8］SHENG Y T,WANG Y L,MEIJER H J G,et al.The heat shock transcription factor PsHSF1 of Phytophthorasojae is required for oxidative stress tolerance and detoxifying the plant oxidative burst［J］.Environ Microbio,2015,17(4):1351-1364.［9］张玉杰,张园园,张华宁,等.小麦热激转录因子基因[STBX]TaHsfA2e[STBZ]特性及耐热性功能初探［J］.作物学报，2018,44(12):1818-1828.［10］WANG X Y,HUANG W,LIU J,et al.Molecular regulation and physiological functions of a novel FaHsfA2c cloned from tall fescue conferring plant tolerance to heat stress［J］.Plant Biotechnol J,2017,15(2):237-248.［11］闫朝辉,李桂荣,扈岩松,等.欧洲葡萄中CIPK基因的克隆及表达分析［J］.园艺学报,2017,44(8):1463-1476.［12］GUO M,LU J P,ZHAI Y F,et al.Genome-wide analysis,expression profile of heat shock factor gene family (CaHsfs) and characterisation of [STBX]CaHsfA2[STBZ] in pepper (Capsicum annuum L.)［J］.BMC Plant Biology,2015,15(1):151.［13］XIN H B,ZHANG H,ZHONG X H,et al.Over-expression of LlHsfA2b,a lily heat shock transcription factor lacking transactivation activity in yeast,can enhance tolerance to heat and oxidative stress in transgenic Arabidopsis seedlings［J］.Plant Cell Tissue and Organ Culture,2017,130(3):617-629.［14］LI C,CHEN Q,GAO X,et al.AtHsfA2 modulates expression of stress responsive genes and enhances tolerance to heat and oxidative stress in Arabidopsis［J］.Life Sci,2005,48:540-550.

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Last Update: 2023-01-17