|Table of Contents|

Cloning，Sequencing and Expression Analysis of Rosa multiflora Rm4CL Gene

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年17
Page:
67-78
Research Field:
Publishing date:

Info

Title:
Cloning，Sequencing and Expression Analysis of Rosa multiflora Rm4CL Gene
Author(s):
CHEN Rui; MA Daocheng; XU Huan; FENG Tiaohong; CHENG Wenhan
(Bioengineering College，Jingchu University of Technology/Hubei Province Special Flower Biological Breeding Engineering Research Center,Jingmen,Hubei 448000)
Keywords:
4CL; Rosa multiflora; cloning; qRT-PCR; bioinformatics analysis
PACS:
-
DOI:
10.11937/bfyy.20220597
Abstract:
Taking the wild rose (Rosa multiflora) as the test material, the genome of the 4CL1α1 gene and the full-length CDS clone were studied by PCR and RT-PCR,in order to provide reference for the anabolic pathway of rose flower color.The results showed that the expression of the 4CL gene family in different tissues of rose and after hormone induction was analyzed by semi-quantitative qRT-PCR. The 4CL1α1 gene was cloned, with gDNA length 4 261 bp and CDS length 1 632 bp, encoding 543 amino acids. Through bioinformatics analysis, it was preliminarily determined to be 4CL or 4CL-like. Through the real-time quantification of fluorescence of 6 genes, it was found that the expression of the 4CL gene family was relatively high in safflower and young thorns. It was preliminarily determined that the Rm4CL gene family in roses plays an important role in anthocyanin synthesis and cell wall thickening.

References:

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Last Update: 2022-11-22