|Table of Contents|

Characteristics and Expression Analysis of VvLBD28 Gene in Grape

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年17
Page:
57-63
Research Field:
Publishing date:

Info

Title:
Characteristics and Expression Analysis of VvLBD28 Gene in Grape
Author(s):
LI Ming1; CAO Hui1; DUAN Gaofei1; LI Gaoqing2; XU Ruirui1
(1.College of Biological and Agricultural Engineering,Weifang University/Key Laboratory of Biochemistry and Molecular Biology in Universities of Shandong,Weifang,Shandong 261061；2.Agricultural Bureau of Shouguang,Weifang,Shandong 262700)
Keywords:
Vitis vinifera; VvLBD28 gene; stress; expression analysis
PACS:
-
DOI:
10.11937/bfyy.20220480
Abstract:
Taking grape as test material,the characteristics of VvLBD28 gene,tissue expression pattern and the effects of drought,salt and temperature stress on VvLBD28 expression were studied by bioinformatics analysis and real-time fluorescent quantitative PCR,in order to provide reference for the study of the stress response process involved in this gene.The results showed that VvLBD28 contained complete leu zipper,belonging to Class I subfamily,highly homologous and high similarity with apple,peach,Arabidopsis and tobacco LBD genes.qRT-PCR analysis showed that the expression level of VvLBD28 was the highest in stem,slightly higher than that in leaf,and the lowest in root of grape.The expression level of VvLBD28 did not change significantly under salt stress and 39 ℃,but decreased significantly under mannitol and 4 ℃,which means VvLBD28 was down-regulated by drought and cold stresses.

References:

[1]卢寰,时振英.高等植物特有的LBD基因的分子生物学功能研究进展[J].植物生理学报,2013,49(9)：833-846.[2]石玉,沈诗雅,张倩茹,等.LBD基因家族研究进展[J].中国细胞生物学学报,2019,41(4)：738-745.[3]SHUAI B,REYNAGA-PEA C G,SPRINGER P S.The lateral organ boundaries gene defines a novel,plant-specific gene family[J].Plant Physiology,2002,129(2)：747-761.[4]YANG Y,YU X,WU P.Comparison and evolution analysis of two rice subspecies LATERAL ORGAN BOUNDARIES domain gene family and their evolutionary characterization from Arabidopsis[J].Molecular Phylogenetics and Evolution,2006,39：248-262.[5]SCHNABLE P S,WARE D,FULTON R S,et al.The B73 maize genome：Complexity,diversity,and dynamics[J].Science,2009,326：1112-1115.[6]MANGEON A,BELL E M,LIN W,et al.Misregulation of the LOB domain gene DDA1 suggests possible functions in auxin signalling and photomorphogenesis[J].Journal of Experimental Botany,2011,62(1)：221-233.[7]王小非,刘鑫,苏玲,等.番茄LBD基因家族的全基因组序列鉴定及其进化和表达分析[J].中国农业科学,2013,46(12)：2501-2513.[8]WANG X,ZHANG S,SU L,et al.A genome-wide analysis of the LBD (lateral organ boundaries domain) gene family in Malus domestica with a functional characterization of MdLBD11[J].PLoS One,2013,8(2)：e57044.[9]CAO H,LIU C Y,LIU C X,et al.Genomewide analysis of the lateral organ boundaries domain gene family in Vitis vinifera[J].Journal of Genetics,2016,95(3)：1-12.[10]GUO B J,WANG J,LIN S,et al.A genome-wide analysis of the ASYMMETRIC LEAVES2/LATERAL ORGAN BOUNDARIES (AS2/LOB) gene family in barley (Hordeum vulgare L.)[J].Journal of Zhejiang University Science B (Biomed & Biotechnol),2016,17(10)：763-774.[11]YANG T,FANG G Y,HE H,et al.Genome-wide identification,evolutionary analysis and expression profiles of LATERAL ORGAN BOUNDARIES DOMAIN gene family in lotus japonicus and medicago truncatula[J].PLoS One,2016,11(8)：e0161901.[12]郑忠凡,张亚利,胡灿,等.辣椒全基因组中LBD转录因子的鉴定与表达分析[J].园艺学报,2016,43(4)：683-694.[13]YU Q,HU S M,DU J C,et al.Genome-wide identification and characterization of the lateral organ boundaries domain gene family in Brassica rapa var. rapa[J].Plant Diversity,2019,42(1):52-60.[14]XU C,LUO F,HOCHHOLDINGER F.LOB domain proteins：Beyond lateral organ boundaries[J].Trends in Plant Science,2016,21(2)：159-67.[15]XU C,CAO H,ZHANG Q,et al.Control of auxin-induced callus formation by bZIP59-LBD complex in Arabidopsis regeneration[J].Nature Plants,2018(4):108-115.[16]LIU S,WANG B,LI X,et al.Lateral organ boundaries domain 19 (LBD19) negative regulate callus formation in Arabidopsis[J].Plant Cell Tissue and Organ Culture,2019,137：485-494.[17]XU B,LI Z,ZHU Y,et al.Arabidopsis genes AS1,AS2,and JAG negatively regulate boundary-specifying genes to promote sepal and petal development[J].Plant Physiology,2008,146(2)：566-575.[18]JUN J H,HA C M,FLETCHER J C.BLADE-ON-PETIOLE1 coordinates organ determinacy and axial polarity in Arabidopsis by directly activating ASYMMETRIC LEAVES2[J].The Plant Cell,2010,22(1)：62-76.[19]ZHOU L L,SHI M Z,XIE D Y.Regulation of anthocyanin biosynthesis by nitrogen in TTG1-GL3/TT8-PAP1-programmed red cells of Arabidopsis thaliana[J].Planta,2012,236(3)：825-837.[20]SHENG L,HU X,DU Y,et al.Non-canonical WOX11-mediated root branching contributes to plasticity in Arabidopsis root system architecture[J].Development,2017,144(17)：3126-3133.[21]HU Y,ZHANG J,JIA H,et al.Lateral organ boundaries 1 is a disease susceptibility gene for citrus bacterial canker disease[J].Proceedings of the National Academy of Sciences of the United States of America,2014,111(4)：521-529.[22]LIU Y,WANG H,JIANG Z,et al.Genomic basis of geographical adaptation to soil nitrogen in rice[J].Nature,2021,590:600-605.[23]郭兆来.拟南芥AtLBD15调控植物干旱胁迫的分子机理研究[D].昆明：昆明理工大学,2019.[24]ZOU X,DU M,LIU Y,et al.CsLOB1 regulates susceptibility to citrus canker through promoting cell proliferation in citrus[J].The Plant Journal,2021,106(4):1039-1057.[25]GRIMPLET J,PIMENTEL D,AGUDELO-ROMERO P,et al.The LATERAL ORGAN BOUNDARIES domain gene family in grapevine：Genome-wide characterization and expression analysis during developmental processes and stress responses[J].Scientific Reports,2017（7）：15968.[26]何红红,马宗恒,张元霞,等.葡萄LBD基因家族的鉴定与表达分析[J].中国农业科学,2018,51(21)：4102-4118.[27]MAJER C,HOCHHOLDINGER F.Defifi ning the boundaries：Structure and function of LOB domain proteins[J].Trends in Plant Science,2011,16(1)：47-52.

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Last Update: 2022-11-22