|Table of Contents|

Identification of Dikaryon-monokaryon Hybrid of Flammulina filiformis by ISSR-SCAR Marker

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年16
Page:
121-127
Research Field:
Publishing date:

Info

Title:
Identification of Dikaryon-monokaryon Hybrid of Flammulina filiformis by ISSR-SCAR Marker
Author(s):
LI Hui; XIA Weiwei; HU Ziyu; WANG Chaojiang; GAO Chunyan
(Institute of Cash Crops，Hebei Academy of Agriculture and Forestry Sciences，Shijiazhuang，Hebei 050051)
Keywords:
Flammulina filiformis; dikaryon-monokaryon mating; ISSR marker; SCAR marker
PACS:
-
DOI:
10.11937/bfyy.20220445
Abstract:
Two strains of Flammulina filiformis were used to create protoplast monokaryons which mating types were determined by mating test.The full compatiable di-mon mating was carried out，and the parents，hybrids and their protoplast monokaryons were used to ISSR-PCR spectrum analysis in order to provide reference for the identification of di-mon hybrid of Flammulina filiformis.The results showed that two types of protoplast monokaryons were obtained for both strains of FC6 and F93 while the ratio of two mating types for monokaryons were different.The hybrid strain of FC693 was obtained by the full compatiable di-mon mating of parent strains of FC6P23 and F93.ISSR spectrum analysis showed that the specific fragment named T3 was detected in both FC693 and FC6P23 while the specific fragment named T9 was detected in both FC693 and F93.These specific fragments were designed by SCAR primers and used to multiplex PCR for hybrid and parent strains.These ISSR-SCAR markers could distinguish the di-mon hybrid accurately and rapidly.

References:

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Last Update: 2022-10-14