|Table of Contents|

Isolation and Identification of Onion Soft Rot Pathogen in Shuangcheng

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年15
Page:
40-44
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Onion Soft Rot Pathogen in Shuangcheng
Author(s):
YU ChongMA JunxiuYAN GengxuanTIAN JiepingYE YangZHANG Shumei
(Institute of Microbiology,Heilongjiang Academy of Sciences,Harbin,Heilongjiang 150010)
Keywords:
onion soft rotpathogenic bacteriaisolationidentification
PACS:
-
DOI:
10.11937/bfyy.20220424
Abstract:
The diseased onions in Shuangcheng onion plantation were used as test materials,the pathogen causing onion soft rot was isolated by tissue isolation and Coch rule,and identified based on morphological,physiological and biochemical characteristics as well as 16S rDNA,gyrB and rpoD sequence analysis,in order to provide reference for the prevention and control of onion soft rot disease.The results showed that a pathogenic bacterium YC1 was isolated from the infected onion tissue.The strain exhibited the strong and widEspectrum pathogenicity.It could also infect cabbage,carrot,pepper,celery and potato tissue.The utilized glucose,fructose,sucrose,malonate,trehalose,sorbitol,mannitoland citrate,did not hydrolyze starch andcellulose.oxidase,H2S,indole and V.P reactions were negative.M.R,contact enzyme,litmus milk,nitrate reduction and gelatin liquefaction were positive.Phylogenetic analysis based on 16S rDNA,gyrB and rpoD sequence showed that the strain had high homology with B.gladioli.The strain YC1 was identified as B.gladioli according to morphological,physiological and biochemical characteristics and 16S rDNA,gyrB and rpoD sequence analysis.

References:

[1]高亮,王冲.京郊林下洋葱高产优质栽培技术[J].北方园艺,2021(21):168-170.[2]马倩,刘欣玲,张新玉.洋葱软腐病的发生与综合防治技术[J].吉林蔬菜,2017(3):24-25.[3]吕和平,魏晖,漆永红,等.甘肃省嘉峪关市洋葱鳞茎软腐病病原鉴定[J].草业学报,2013,22(4):153-159.[4]王勇,高璐瑶,张郑,等.黑龙江省洋葱主产区鳞茎致腐病原分离鉴定[J].东北农业大学学报,2018,49(2):48-54.[5]陈少先,刘悦,曾瑶英,等.鹰嘴桃采后病原菌的分离与鉴定[J].北方园艺,2021(17):108-118.[6]晋知文,宋加伟,谢学文,等.芹菜细菌性软腐病病原的分离与鉴定[J].植物病理学报,2016,46(3):304-312.[7]赵欢欢,张元元,张映曈,等.娃娃菜(Brassica campestris)软腐病病原菌的分离与鉴定[J].食品工业科技,2021,42(18):106-111.[8]孙齐英,夏明.武汉蔬菜市场大白菜软腐病新病原菌鉴定[J].江苏农业科学,2020,48(15):141-143.[9]李晓颖,宋莉,田宇,等.芹菜腐烂病原菌QC02的鉴定及其生物学特性[J].农业生物技术学报,2018,26(10):1778-1786.[10]杜迅,胡宜亮,何蔚荭,等.软腐白菜细菌群落结构多样性与生长环境的相关性[J].微生物学报,2011,51(12):1639-1645.[11]李晓颖,田宇,赵亮,等.京郊快菜细菌性软腐病病原鉴定[J].华北农学报,2018,33(3):63-70.[12]黄宇飞.胡萝卜软腐果胶杆菌巴西亚种全基因组解析及致病相关基因功能研究[D].沈阳:沈阳农业大学,2019.[13]魏晖.洋葱鳞茎细菌性软腐病病原及农业措施防控技术[D].兰州:甘肃省农业科学院,2010.[14]ZHANG L,GAO W N,YIN Y P.Discrimination of B.gladioli pv.alliicola and B.cepacia complex using the gyrB gene of B.gladioli pv.alliicola[J].Experimental and Therapeutic Medicine,2019(17):1870-1876.[15]BENJAMIN D,TAWATCHAI T,DAVID Z,et al.Multimodal molecular imaging and identification of bacterial toxins causing mushroom soft rot and cavity disease[J].Chem Bio Chem,2021(22):2901-2907.[16]YOU C P,XIANG M M,ZHANG X,et al.First report of bacterial leaf streak of strelitzia reginae caused by B.cepacia[J].Plant Disease,2013,98(5):682.[17]YUKIKO M,HIROSUKE S,AKINORI K,et al.Phylogenetic study and multiplex PCR based detection of B.plantarii,B.glumae and B.gladioli using gyrB and rpoD sequences[J].International Journal of Systematic and Evolutionary Microbiology,2006,56:1031-1038.

Memo

Memo:
-
Last Update: 2023-09-19