|Table of Contents|

Isolation and Identification of Onion Soft Rot Pathogen in Shuangcheng

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2023年15
Page:
40-44
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Onion Soft Rot Pathogen in Shuangcheng
Author(s):
YU Chong; MA Junxiu; YAN Gengxuan; TIAN Jieping; YE Yang; ZHANG Shumei
(Institute of Microbiology，Heilongjiang Academy of Sciences，Harbin,Heilongjiang 150010)
Keywords:
onion soft rot; pathogenic bacteria; isolation; identification
PACS:
-
DOI:
10.11937/bfyy.20220424
Abstract:
The diseased onions in Shuangcheng onion plantation were used as test materials，the pathogen causing onion soft rot was isolated by tissue isolation and Coch rule，and identified based on morphological，physiological and biochemical characteristics as well as 16S rDNA，gyrB and rpoD sequence analysis,in order to provide reference for the prevention and control of onion soft rot disease.The results showed that a pathogenic bacterium YC1 was isolated from the infected onion tissue.The strain exhibited the strong and widEspectrum pathogenicity.It could also infect cabbage，carrot，pepper，celery and potato tissue.The utilized glucose，fructose，sucrose，malonate，trehalose，sorbitol，mannitoland citrate，did not hydrolyze starch andcellulose.oxidase，H2S,indole and V.P reactions were negative.M.R，contact enzyme，litmus milk，nitrate reduction and gelatin liquefaction were positive.Phylogenetic analysis based on 16S rDNA，gyrB and rpoD sequence showed that the strain had high homology with B.gladioli.The strain YC1 was identified as B.gladioli according to morphological，physiological and biochemical characteristics and 16S rDNA，gyrB and rpoD sequence analysis.

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Last Update: 2023-09-19