|Table of Contents|

Technical System of Rapid Propagation of Tissue Culture in vitro of Photinia fraseri

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年14
Page:
49-56
Research Field:
Publishing date:

Info

Title:
Technical System of Rapid Propagation of Tissue Culture in vitro of Photinia fraseri
Author(s):
SUN Lizhong1; CHEN Jing2; HU Xiujin3; YANG Yanmin1; LIU Tong4; HAI Xinmin5
(1.College of Landscape Architecture,Shangqiu University,Shangqiu,Henan 476000；2.Education Group of Xinglong Township，Xiangfu District，Kaifeng，Henan 475100；3.Lankao Yellow River Bureau，Lankao，Henan 475300；4.College of Life Sciences，Shihezi University,Shihezi,Xinjiang 832003；5.College of Foreign Languages，Shangqiu University，Shangqiu，Henan 476000)
Keywords:
Photinia fraseri; first generation culture; proliferation culture; rooting culture
PACS:
-
DOI:
10.11937/bfyy.20220397
Abstract:
The most common Photinia fraseri ‘Red Robin’ were used as experiment materials，this experiment was carried out by cutting the stem segments with buds and the top bud segments as explants to cultivate tissue.While it focused on the effects of different hormone combinations on calli in primary culture，of different hormone combinations on adventitious buds in proliferation culture and of different hormone combinations on rooting in rooting culture，in order to screen out the best combination of hormone concentration in different stages of tissue culture of Photinia fraseri in vitro and provide reference for large-scale breeding of Photinia fraseri.The results showed that the most suitable medium for the first generation culture of Photinia fraseri was MS+0.4 mg?L-1 BA+0.1 mg?L-1 IBA.According to the orthogonal design experiment，the optimal phytohormone combination of medium for proliferation culture of Photinia fraseri was MS+1.6 mg?L-1 BA+0.4 mg?L-1 KT+0 mg?L-1 NAA （That was，no NAA）+0.2 mg?L-1 IBA.The optimal medium for rooting culture of Photinia fraseri was 1/2MS+0.2 mg?L-1 IBA.

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Last Update: 2022-09-06