|Table of Contents|

Polyploid Induction and Identification of Two Wild Clematis

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年18
Page:
52-59
Research Field:
Publishing date:

Info

Title:
Polyploid Induction and Identification of Two Wild Clematis
Author(s):
LIU ZhijieZHENG YanNIU WeikangWANG XinLI MingyangLIU Dongyun
(College of Landscape Architecture and Tourism,Hebei Agricultural University,Baoding,Hebei 071000)
Keywords:
Clematis urophylla Franch.Clematis heracleifolia DC.polyploidcolchicinephotosynthetic index
PACS:
-
DOI:
10.11937/bfyy.20214981
Abstract:
The germinating seeds of wild Clematis urophylla Franch. and Clematis heracleifolia DC.in Hebei Province were used as experimental materials,colchicine was used for polyploid induction,the mutagenesis effects of different treatment combinations on two kinds of Clematis were studied,in order to obtain polyploid materials of Clematis.The results showed that the effect of colchicine treatment on plant morphology and seedling formation was remarkable.It was found that 0.1% colchicine treatment had a certain promoting effect on plant growth,and 0.3% colchicine showed a strong inhibitory effect.The chromosome doubling of the mutant strain was determined by chromosome counting and flow cytometry,and combined with the growth status of the plant,it was found that the mutagenesis effect was the best when the concentration of colchicine was 0.2% and the treatment time was 48 hours.The chlorophyll content increased,intercellular carbon dioxide concentration decreased,stomatal conductance,net photosynthetic rate and transpiration rate increased,which proved that the photosynthesis of mutant was enhanced.The research results could provide reference and theoretical support for the polyploid breeding of Clematis.The polyploid materials of Clematis are expected to be popularized as a new variety after further cultivation.

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Last Update: 2022-11-29