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Optimization of Production Conditions and Antioxidant Activity of Total Flavonoids From Glycyrrhiza uralensis Fisch.Suspension Cells

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年14
Page:
93-101
Research Field:
Publishing date:

Info

Title:
Optimization of Production Conditions and Antioxidant Activity of Total Flavonoids From Glycyrrhiza uralensis Fisch.Suspension Cells
Author(s):
LI Wei1; 2; ZHANG Dongxiang1; LIU Lijie1; LYU Qing1; YUAN Mengyao1; ZHAO Yue1
(1.College of Life Sciences and Agriculture and Forestry，Qiqihar University/Heilongjiang Provincial Key Laboratory of Resistance Gene Engineering and Protection of Biodiversity in Cold Areas,Qiqihar，Heilongjiang 161006；2.Branch of Animal Husbandry and Veterinary，Heilongjiang Academy of Agricultural Sciences，Qiqihar，Heilongjiang 161005)
Keywords:
Glycyrrhiza uralensis Fisch.; suspension cells; response surface; total flavonoids; antioxidant
PACS:
-
DOI:
10.11937/bfyy.20214495
Abstract:
Taking Glycyrrhiza uralensis suspension cells as experimental material，the effects of hydrolyzed casein，2,4-D and 6-BA on the yield of total flavonoids from G.uralensis suspension cells were studied by response surface methodology，in order to obtain the optimal culture conditions for the production of total flavonoids from G.uralensis suspension cells，provide reference for production.The results showed that when the suspension cell culture medium was MS liquid medium+CH 398.608 mg?L-1+2,4-D 1.044 mg?L-1+6-BA 1.325 mg?L-1，the maximum yield of total flavonoids predicted by the model was 1.324%，and the yield of total flavonoids in the validation test was 1.286%±0.03%，which was less different from the predicted value.The crude extract of total flavonoids from G.uralensis suspension cells had certain antioxidant capacity，and the total antioxidant capacity was better than vitamin C.

References:

[1]ZHANG H C,LIU J M,LU H Y,et al.Enhanced flavonoid production in hairy root cultures of Glycyrrhiza uralensis Fisch.by combining the over-expression of chalcone isomerase gene with the elicitation treatment[J].Plant Cell Rep,2009,28(8):1205-1213.[2]YANG Y,HE F,YU L J.Dynamics analyses of nutrients consumption and flavonoids accumulation in cell suspension culture of Glycyrrhiza inflata[J].Biologia Plantarum,2008,52(4):732-734.[3]WANG L,YANG R,YUAN B,et al.The antiviral and antimicrobial activities of licorice，a widely-used Chinese herb[J].Acta Pharm Sin B,2015,5(4):310-315.[4]杨世海,刘晓峰,沈昕,等.甘草Ri质粒转化及不同理化因子对甘草毛状根生长的影响[J].中国中药杂志,2006,31(11):4.[5]WANG J,GAO W,ZHANG L,et al.Establishment and quality assessment of tissue cultures in Glycyrrhiza uralensis Fisch[J].Appl Biochem Biotechnol,2013,169(2):588-594.[6]PARK Y J,KIM J K,PARK S U.Yeast extract improved biosynthesis of astragalosides in hairy root cultures of Astragalus membranaceus[J].Prep Biochem Biotechnol,2021,51(5):467-474.[7]MURTHY H N,LEE E J,PAEK K Y.Production of secondary metabolites from cell and organ cultures:Strategies and approaches for biomass improvement and metabolite accumulation[J].Plant Cell Tissue & Organ Culture,2014,118(1):179-186.[8]A E S,A H M,B E N,et al.Hepatoprotective properties and biotransformation of berberine and berberrubine by cell suspension cultures of Dodonaea viscosa and Ocimum basilicum[J].South African Journal of Botany,2015,97:191-195.[9]RANI D,MEELAPH T,de EKNAMKUL W,et al.Yeast extract elicited isoflavonoid accumulation and biosynthetic gene expression in Pueraria candollei var. mirifica cell cultures[J].Plant Cell Tissue and Organ Culture,2020,141(1):112-119.[10]MKEL M.Experimental design and response surface methodology in energy applications:A tutorial review[J].Energy Conversion and Management,2017,151:630-640.[11]BINDES M,REIS M,CARDOSO V L,et al.Ultrasound-assisted extraction of bioactive compounds from green tea leaves and clarification with natural coagulants (chitosan and Moringa oleífera seeds)[J].Ultrasonics Sonochemistry,2019,51:111-119.[12]XIE J H,DONG C J,NIE S P,et al.Extraction，chemical composition and antioxidant activity of flavonoids from Cyclocarya paliurus (Batal.) Iljinskaja leaves[J].Food Chem,2015,186:97-105.[13]ZHU Y,YU X,GE Q,et al.Antioxidant and anti-aging activities of polysaccharides from Cordyceps cicadae[J].Int J Biol Macromol,2020,157(15):394-400.[14]YANG Y,HE F,YU L,et al.Flavonoid accumulation in cell suspension cultures of Glycyrrhiza inflata Batal. under optimizing conditions[J].Z Naturforsch C J Biosci,2009,64(1/2):68-72.[15]VILLEGAS A,ARIAS J P,ARAGN D,et al.First principle-based models in plant suspension cell cultures:A review[J].Crit Rev Biotechnol,2017,37(8):1077-1089.[16]FU Y,CHEN J,LI Y J,et al.Antioxidant and anti-inflammatory activities of six flavonoids separated from licorice[J].Food Chem,2013,141(2):1063-1071.

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Last Update: 2022-09-08