|Table of Contents|

Determination of Total Alkaloids in Aconitum leucostomum by UV-vis Spectrophotometry

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年12
Page:
94-98
Research Field:
Publishing date:

Info

Title:
Determination of Total Alkaloids in Aconitum leucostomum by UV-vis Spectrophotometry
Author(s):
Abulimiti?YUMAIER1; Korbanjhon?BRAD2
(1.College of Chemistry and Environmental Science,Kashi University,Kashi,Xinjiang 844007;2.School of Chemistry and Environmental Sciences,Yili Normal University,Yining,Xinjiang 835000)
Keywords:
Aconitum leucostomum; total alkaloids; spectrophotometry; ultrasonic extraction; content determination
PACS:
-
DOI:
10.11937/bfyy.20214481
Abstract:
Different parts of Aconitum leucostomum were used as test materials and extracted by anhydrous ethanol ultrasonic method,the absorbance was measured with a spectrophotometer at a wavelength of 231 nm,and the total alkaloids were calculated.A method for the determination of total alkaloids in different parts of Aconitum leucostomum from different origins was established,in order to provide reference for the development and utilization of Aconitum carmichaeli.The results showed that aconitine had a good linear relationship in the concentration range of 0.000 4-0.002 0 mg?L-1,the regression equation was Y=0.179 5X+0.511 72,r= 0.999 4,the precision was good,RSD=3.2% (n=3).The sample solution was stable within 2 hours fter color development,RSD=2.14% (n=3).The reproducibility of this method was good,RSD=1.49% (n=3).The average recovery was 99.886%,RSD=1.27% (n=3),the average content was 0.063 7 mg?g-1,RSD=0.716% (n=3).

References:

[1]李娅萍,田颂九.乌头类药物的化学及分析方法概况[J].中国中医杂志,2008,26(10):659-662.[2]符华林.我国乌头属药用植物的研概况[J].中药材,2006,27(2):149-154.[3]巴雷,王德利,高莹.松嫩平原优势种羊草与其主要伴生种芦苇空间分布格局分析[J].草业学报,2015,5(11):253-267.[4]罗开雷,安沙舟,李学贤,等.有毒植物白喉乌头化学防除的初步研究[J].新疆农业科学,2012,43(5):310-345.[5]谢海辉,韦璧瑜.高乌头的化学成分及其药理作用研究进展[J].天然产物研究与开发,2010,12(9):232-233.[6]许敬人,房敏,严隽陶.手法治疗颈痛、头痛的临床及其机制[J].颈腰痛杂志，2000,21(1)：67-69.[7]程丽丽,许妍妍,NYIRMIGABO E,等.一测多评法测定乌头类药材中生物碱[J].现代药物与临床,2013,28(4):528-534.[8]中国医学科学院药物研究所.中草药有效成分的研究[M].北京:人民卫生出版社,2019.[9]沈映君. 中药药理学[M]. 上海:上海科学技术出版社,2010.[10]郭孝武,张福成,林书玉,等.超声提取对黄连素提出率的影响[J].中国中药杂志,1995(11):673-675，703. [11]王玉华,王伟,刘笑融,等．交流示波极谱滴定法测定草乌及那如-3中总乌头生物碱含量[J]．中草药,1992,23(10):521-522.[12]史巧霞,朱应怀,靳子明.紫花高乌头药材的TLC鉴别及主要生物碱的测定[J].华西药学杂志,2019,34(4):375-378．[13]王朝虹,何毅,张继宗,等．生物检材中乌头碱的GC/MS分析[J]．中国法医学杂志,2003(3)：145-146.[14]杨海宁,文瑾,孙筱林,等．高效液相色谱法测定生附子中3种双酯型生物碱含量[J].医药导报,2019,38(11)：1459-1462．

Memo

Memo:
-
Last Update: 2022-08-26