|Table of Contents|

Effects of Different Juncao on Solid Cultured Mycelium of Antrodia camphorata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年08
Page:
108-115
Research Field:
Publishing date:

Info

Title:
Effects of Different Juncao on Solid Cultured Mycelium of Antrodia camphorata
Author(s):
LI Jing1XIA Shuning2ZHANG Dai2XIE Jing13LU Yamei2LIN Zhanxi1
(1.National Engineering Research Center of Juncao,Fujian University of Agriculture and Forestry,Fuzhou,Fujian 350002;2.College of Life Science,Fujian University of Agriculture and Forestry,Fuzhou,Fujian 350002;3.College of Agriculture and Biology Technology,Hunan University of Humanities,Science and Technology,Loudi,Hunan 417000)
Keywords:
Antrodia camphorataJuncaogrowth ratetriterpenesextracellular enzymes
PACS:
-
DOI:
10.11937/bfyy.20214350
Abstract:
The culture medium of Antrodia cinnamomea mycelium was prepared with the extracts of five kinds of Juncao,including Cymbopogon citratus,Clinacanthus nutans,Pennisetum giganteum,Pennisetum purpureum and Pennisetum purpureum Schumab cv.Purple.The mycelium growth rate,triterpene,amino acids,extracellular enzyme activity and the key genes expression of triterpene synthesis pathway were used to screen out the culture medium promoting the mycelium growth and active substances accumulation of Antrodia cinnamomea,in order to provide reference to solve the problems of high cost and long time-consuming of artificial culture of Antrodia cinnamomea in the future.The extracts of Cymbopogon citratus and Clinacanthus nutans were significantly promoted the growth of mycelium of Antrodia camphorata,and the cultivation time was 12 days shorter than the control.The promotion effect of different mycorrhizal extracts on the triterpene content of Antrodia camphorata were as follows,XMC>JJC>YDC>ZSXC>XC,which were significantly different with the control;Cymbopogon citratus significantly promoted the synthesis of laccase and glutamate synthase,and significantly promoted the expression of AcHMGS,AcHMGR and AcSE.Cymbopogon citratus could be also used as mycelia culture medium of Antrodia camphorata to promote the growth and the accumulation of triterpene content.

References:

[1]CHANG T T,CHOU W N.Antrodia cinnamomea sp.nov.on Cinnamomum kanehirai in Taiwan[J].Mycological Research,1995,99(6):756-758.[2]CHANG T T,CHOU W N.Antrodia cinnamomea reconsidered and A.salmonea sp.nov.on Cunninghamia konishii in Taiwan[J].Botanical Bulletin of Academia Sinica,2004,45(4):347-352.[3]LU M C,EL-SHAZLY M,WU T Y,et al.Recent research and development of Antrodia cinnamomea[J].Pharmacol Ther,2013,139(2):124-156.[4]姚秀英.牛樟芝人工培养条件的初步研究[D].济南:山东师范大学,2011.[5]王谦,胡卫静.大型食药用真菌深层发酵研究进展[J].食品安全质量检测学报,2016,7(3):1240-1246.[6]LI Z W,KUANG Y,TANG S N,et al.Hepatoprotective activities of Antrodia camphorata and its triterpenoid compounds against CCl4-induced liver injury in mice[J].Journal of Ethnopharmacology,2017,206:31-39.[7]蔡为明,金群力,蔡青松,等.牛樟芝人工培养及活性成分与药理作用的研究进展[J].食药用菌,2015(1):17-23.[8]冯路瑶,程显好,董洪新,等.不同中药提取物对牛樟芝生长和胞内三萜产物形成的影响[J].中国食用菌,2018,37(2):42-46.[9]周璇,夏永军,刘胜男,等.不同培养方式对牛樟芝活性组分的影响[J].工业微生物,2017,47(2):18-23.[10]MA T W,LAI Y,YANG F C.Enhanced production of triterpenoid in submerged cultures of Antrodia cinnamomea with the addition of citrus peel extract[J].Bioprocess & Biosystems Engineering,2014,37(11):2251-2261.[11]赵能,原晓龙,陈剑,等.不同碳氮源对牛樟芝菌丝体生长的影响[J].西部林业科学,2016,45(4):7-12.[12]林占熺,林冬梅.黄河菌草生态治理[M].福州:福建科学技术出版社,2020.[13]李晶.牛樟芝MVA途径中基因克隆及转录组、代谢组研究[D].福州:福建农林大学,2016.[14]李晶,林雄杰,童金华,等.牛樟芝ITS序列分析及液体发酵菌丝体总三萜含量测定[J].西南农业学报,2015,28(6):2649-2654.[15]张瑞,吴萼,殷晓浦,等.倍半萜合酶催化机制的研究进展[J].生命的化学,2018,38(1):135-143.[16]徐圣东,周金洋,王丽,等.猴头菌和金针菇漆酶对不同染料的降解[J].菌物学报,2021,40(6):1525-1537.[17]广东东阳光药业有限公司,乳源南岭好山好水食品有限公司.牛樟芝液体培养基及其制备方法[P].中国:CN201810005885.2,2018-01-03.[18]LEATHAM F G.Extracellular enzymes produced by the cultivated mushroom Lentinus edodes during degradation of a lignocellulosic medium[J].Appl Environ Microbiol,1985,50(4):859-867.[19]贺春玲,李明,田景花,等.葡萄木屑栽培香菇配方筛选及胞外酶活性研究[J].中国食用菌,2019,38(7):43-49.[20]冯作山,邹亚杰,胡清秀.白灵侧耳栽培过程中胞外酶和呼吸酶活及离子流速的研究[J].菌物学报,2014,33(2):341-354.[21]张国利,田雪梅,宋爱荣.八个樟芝菌株液体培养两种胞外酶活性的测定[J].中国食用菌,2009,28(2):43-45.[22]吴周斌,张健,王佳敏,等.不同培养料对真姬菇胞外酶活性的影响[J].北方园艺,2015(8):145-149.[23]方洪枫,赵光辉,吴汉琼,等.平菇胞外酶研究进展综述[J].食药用菌,2020,28(4):4.[24]LU M Y J,FAN W L,WANG W F,et al.Genomic and transcriptomic analyses of the medicinal fungus Antrodia cinnamomea for its metabolite biosynthesis and sexual development[J].Proceedings of the National Academy of Sciences of the United States of America,2014,111(44):E4743.[25]朱静,岳思宁,陈琛,等.谷氨酸合酶在灵芝中生物学功能的研究[J].南京农业大学学报,2019,42(6):1073-1079.[26]SHIAO M S.Natural products of the medicinal fungus Ganoderma lucidum:Occurrence,biological activities,and pharmacological functions[J].Chemical Record,2010,3(3):172-180.[27]KUMARI P,MEENA A.Application of enzyme-mediated cellulose nanofibers from lemongrass waste for the controlled release of anticancer drugs[J].Environmental Science and Pollution Research,2020(28):46343-46355.[28]YOUNIS N S,ELSEWEDY H S,SOLIMAN W E,et al.Geraniol isolated from lemon grass to mitigate doxorubicin-induced cardiotoxicity through Nrf2 and NF-κB signaling[J].Chemico-Biological Interactions,2021,347(6):109599.[29]CELINA A,RAHMAWATI D,PERMANA T.Application of lemongrass essential oil as a natural preservative agent for pineapple juice[J].Iconiet Proceeding,2019,2(2):69-78.[30]PARAGODAARACHCHI Y L,WICKRAMARACHCHI P A S R,SUBASINGHE U G C P.In-vitro evaluation of antifungal efficacy of lemongrass oil containing chitosan microcapsules against Aspergillus flavus[C].Asian Symposium on Medicinal Plants,Spices and Other Natural Products XVI,2018.[31]苏荣镇,夏继蓉,温燕龙,等.香茅草提取物对鸡肉肠品质及抗氧化性能的影响[J].食品安全质量检测学报,2021,12(16):6332-6336.[32]黄国平,刘岩,徐彩虹,等.香茅草精油在脂溶性食品体系中抗氧化活性研究[J].农业机械,2012(12):57-59.[33]许智萍,范源洪,何璐,等.云南香茅草精油含量及主要化学成分分析[J].热带农业科学,2020,279(11):118-123.[34]ILORI N,LIEW X Q,FANG C M.The anti-inflammatory properties of Acanthus ebracteatus,Barleria lupulina and Clinacanthus nutans:A systematic review[J].Molecular Biology Reports,2020,47(12):1-12.[35]桑圣刚,张瑞,徐亚晨,等.基于网络药理学的忧遁草治疗乙型肝炎的成分分析及作用机制研究[J].中南民族大学学报(自然科学版),2019(3):383-388.[36]林占熺.菌草学概论[M].北京:中国农业出版社,2019.

Memo

Memo:
-
Last Update: 2022-07-08