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In Vitro Tissue Culture Technology of Leaves of Petrocosmea qinlingensis

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年10
Page:
70-76
Research Field:
Publishing date:

Info

Title:
In Vitro Tissue Culture Technology of Leaves of Petrocosmea qinlingensis
Author(s):
HU Xuanping1; 2; 3; JIANG Jinglong1; 2; 3; WANG Qi1; 2; 3; LI Yun4
(1.College of Biology Sciences and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723000；2.Shaanxi Province Key Laboratory of Bio-resources,Hanzhong,Shaanxi 723000；3.Qinba State Key Laboratory of Biological Resources and Ecological Environment,Shaanxi University of Fechnology,Hanzhong,Shaanxi 723000；4.Hanzhong City Wildlife Conservation and Management Station,Hanzhong,Shaanxi 723000)
Keywords:
Petrocosmea qinlingensis; leave; differentiation; multiplication; plant regeneration
PACS:
-
DOI:
10.11937/bfyy.20214303
Abstract:
Taking leaves of Petrocosmea qinlingensis as test materials,the effects of explants disinfection,primary culture,subculture and differentiation of callus,adventitive bud multiplication,rooting and seedling training on regeneration of Petrocosmea qinlingensis were studied by using in vitro tissue culture method,in order to provide reference for the establishment of rapid propagation system of Petrocosmea qinlingensis.The results showed that optimal disinfection method was treatment with 0.1% mercuric chloride 2 minutes and wash with sterile water 7—8 times.Best medium for callus induction and subculture was MS+2.0 mg?L-1 IBA.Suitable medium for callus differentiation was MS+0.1 mg?L-1 6-BA+0.2 mg?L-1 NAA+0.1 mg?L-1IBA.The results of orthogonal experiment indicated that the proper condition for adventitious buds induction was MS+0.1 mg?L-1 6-BA+0.1 mg?L-1 IBA+0.1 mg?L-1 ZT and maximum multiplication coefficient up to 14.06.Optimum rooting and growing cultivation conditions was substrate of perlite,vermiculite and peat(2∶1∶1)+MS nutrient solution (1 mL)+200 mg?L-1 IBA (0.5 mL).

References:

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Last Update: 2022-07-04