|Table of Contents|

Cloning and Functional Analysis of CtARF57 and CtIAA12 From Calanthe triplicata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年09
Page:
80-87
Research Field:
Publishing date:

Info

Title:
Cloning and Functional Analysis of CtARF57 and CtIAA12 From Calanthe triplicata
Author(s):
WAN Zhiting; QIN Si; WU You; CHEN Lunyan; WU Shasha; ZHAI Junwen
(College of Landscape Architecture，Fujian Agriculture and Forestry University/Key Laboratory of National Forestry and Grassland Administration for Orchid Conservation and Utilization，Fuzhou，Fujian 350002)
Keywords:
Calanthe triplicata; petal spur; cloning; auxin signal
PACS:
-
DOI:
10.11937/bfyy.20214183
Abstract:
The petal spur of Calanthe triplicata at different development stages was used as the test materials.Two genes that expressed differentially during the auxin signal transduction pathway,namely CtARF57 and CtIAA12,were cloned from petal spur of Calanthe triplicata based on transcriptome data and analyzed by bioinformatics,in order to investigate the expression characteristics and functions of auxin related response factors (ARF and Aux/IAA) in Calanthe triplicata,and to reveal the development mechanism of petal spur.The results showed that the full length of cDNA were 3 054 bp and 1 124 bp,encoding 911 and 177 amino acids,respectively.Homology analysis showed that the amino acid sequences of the two genes had high homology similarity with Dendrobium catenatum,and the similarity was more than 84%．Conserved domain analysis showed that CtARF57 protein had B3 domain,Auxin_resp domain and Aux/IAA domain;CtIAA12 contained the typical Aux/IAA protein DomainⅠ,Ⅱ,Ⅲ and Ⅳ．The results of real-time fluorescence quantitative PCR showed that the transcriptional level was induced by auxin,and the expressions of the two genes were significantly different at different stages．The expression levels of the genes were the highest at the last stage of petal spur development,and were lower in the first and second periods,which was consistent with the trend of FPKM value of the transcriptomic.The above results preliminarily suggested that two response factor genes were involved in the auxin signal transduction pathway to regulate the morphogenesis,growth and metabolism of Calanthe triplicata in petal spur of the garden flower.

References:

[1]TSAI J Y,WANG T T,HUANG P L,et al．Effects of developmental stages on postharvest performance of White Crane Orchid (Calanthe triplicata) inflorescences[J].Scientia Horticulturae,2021,281:109988.[2]蒋明,吴棣飞,鲍洪华,等.虾脊兰属11种药用植物rDNA ITS的克隆与序列分析[J].中草药,2018,49(14):3369-3375.[3]罗远华,黄敏玲,叶秀仙,等.三褶虾脊兰无菌播种快繁技术研究[J].南方农业学报,2011,42(7):708-711.[4]伍小燕,田辉,叶晶,等.壮药万筋藤的显微特征研究[J].广西医学,2010,32(9):1134-1135.[5]谷小红,郭宝林,田景,等.植物生长调节剂在药用植物生长发育和栽培中的应用[J].中国现代中药，2017,19(2)：295-305,310.[6]李玲,肖浪涛.植物生长调节剂应用手册[M].北京:化工出版社,2013.[7]JUNG J H,LEE M,PARK C M.A transcriptional feedback loop modulating signaling crosstalks between auxin and brassinosteroid in Arabidopsis[J].Molecules and Cells,2010,29(5):449-456.[8]VARAUD E，BRIOUDES F，SZECSI J，et al．Auxinresponsefactor 8 regulates Arabidopsis petal growth by interacting with the bHLH transcription factor BIGPETALp[J].Plant Cell,2011,23(3):973-983.[9]WEIJERS D，FRIML J．SnapShot:Auxin signaling and transport[J].Cell,2009,136(6):1172.[10]余姝姝,周俊琴,卢梦琪,等.油茶3个ARF基因的克隆及表达分析[J].植物生理学报,2021,57(5):1151-1162.[11]潘君飞,彭丽云,赵春丽,等.苋菜amaARF6基因克隆及其外源激素处理下在幼苗中响应分析[J].东北农业大学学报,2018,49(5):24-32.[12]SARBOTTAM P,SHRESTHA S K,BRAD B,et al.Protein-protein interaction and gene co-expression maps of ARFs and Aux/IAAs in Arabidopsis[J].Frontiers in Plant Science,2014(5):744.[13]周成城,谢德金,万志庭,等.巴戟天苯丙烷类代谢途径相关基因的克隆与表达[J].应用与环境生物学报,2021,27(1):158-166.[14]庞天虹,钱婕妤,付建新,等.桂花己糖激酶基因家族成员的序列及表达分析[J].浙江农林大学学报,2021,38(2):225-234.[15]徐新然,陈向东,张薇薇,等.生长素调节药用植物生长发育的研究进展[J].中国现代中药,2016,18(12):1675-1678.[16]CHENG Y F,ZHAO Y D.A role for auxin in flower development[J].Journal of Integrative Plant Biology,2007,49(1):99-104.[17]GUILFOYLE T J,ULMASOV T,HAGEN G.The ARF family of transcription factors and their role in plant hormone-respon-sive transcription[J].Cellular and Molecular Life Sciences,1998,54(7):619-627.[18]GUILFOYLE T J,HAGEN G.Auxin response factors[J].Current Opinion in Plant Biology,2007,10(5):453-460.[19]李俊男,燕晓杰,李枢航,等.植物AUX/IAA基因家族研究进展[J].中国农学通报,2018,34(15):89-92.[20]苏江,郭天玮,季孔庶.马尾松Aux/IAA家族相关基因克隆及初步分析[J].分子植物育种,2015,13(8):1822-1830.[21]HONG Y,SOLER M,MILA I,et al.Genome-wide characterization and expression profiling of the auxinresponse factor(ARF) gene family in Eucalyptus grandis[J].PLoS One,2014,9(9):e108906.[22]ZHANG R,MIN Y,HOLAPPA Y D,et al.A role for the auxin response factors ARF6 and ARF8 homologs in petal spur elongation and nectary maturation in Aquilegia[J].New Phytologist,2020,227(5):1392-1405.[23]张丽丽.三个棉花生长素信号转导途径相关基因的克隆与鉴定[D].南京：南京农业大学,2010.[24]ZHANG S Z,WU T,LIU S J,et al.Disruption of OsARF19 is critical for floral organ development and plant architecture in rice (Oryza sativa L.)[J].Plant Mol Biol Rep,2016,34(4):748-760.[25]杨冬寅.菊花生长素响应蛋白Aux/IAA编码基因CmIAA1的克隆及功能鉴定[D].南京：南京农业大学,2012.[26]沈立明,钟惠,朱雅婷,等.温度胁迫下4种广义虾脊兰属植物的光合特性[J].森林与环境学报,2021,41(1):60-65.

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Last Update: 2022-06-27