|Table of Contents|

Identification and Biological Characters of Two Wild Lentinula Strains

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年06
Page:
107-113
Research Field:
Publishing date:

Info

Title:
Identification and Biological Characters of Two Wild Lentinula Strains
Author(s):
FANG Zhirong12QING Yuan2HE Xiaolan3NAIGE Riyou4
(1.Resource and Environment Department,Xichang University,Xichang,Sichuan 615013;2.Panxi Crops Research and Utilization Key Laboratory of Sichuan Province,Xichang University,Xichang,Sichuan 615013; 3.Soil and Fertilizer Institute,Sichuan Academy of Agricultural Sciences,Chengdu,Sichuan 610066; 4.Education,Sports and Science and Technology Bureau of Puge County,Puge,Sichuan 615302)
Keywords:
Lentinula edodes ITS sequence analysis biological characteristic
PACS:
-
DOI:
10.11937/bfyy.20213873
Abstract:
Taking the fruiting bodies of wild Lentinula fungus collected from the mountain in Puge county,Sichuan Province as the test material,strains HG-2 and BG-1 were obtained by tissue isolation.Strains HG-2 and BG-1 were identified by DNA sequences.The effects of different carbon sources,nitrogen sources,temperature and pH on the growth of HG-2 and BG-1 strains were studied,in order to provide a reference for the utilization of wild Lentinula resources.The results showed that the two wild strains were Lentinula edodes.The optimum carbon source for the mycelial growth of HG-2 and BG-1 strains was corn meal,the optimum nitrogen source was yeast extract; the optimal cultivation temperature was 25 ℃,and the optimal pH was 6.0.

References:

[1]International Mycological Association.Mycobank database fungal databases,nomenclature and species banks[EB/OL].http://www.mycobank.org.(2017-11-06)[2021-17-06].[2]闵庆文,柳林飞.浙江庆元香菇文化系统[M].北京:中国农业出版社,2017.[3]何晋浙,孙培龙,朱建标,等.香菇营养成分的分析[J].食品研究与开发,1999,20(6):44-46.[4]郑世杰,张蕊,杨树德,等.香菇中麦角钙化甾醇含量的测定及其在人体内的传输机制[J].菌物学报,2019,38(5):739-752.[5]DUBOST N J,OU B,BEELMAN R B.Quantification of polyphenols and ergothioneine in cultivated mushrooms and correlation to total antioxidant capacity[J].Food Chem,2007,105(2):727-735.[6]REIS F S,MARTINS A,BARROS L,et al.Antioxidant properties and phenolic profile of the most widely appreciated cultivated mushrooms:A comparative study between in vivo and in vitro samples[J].Food Chem Toxicol,2012,50(5):1201-1207.[7]CHANG S T,WASSER S P.The role of culinary-medicinal mushrooms on human welfare with a pyramid model for human health[J].Int J Med Mushrooms,2012,14(2):95-134.[8]POUCHERET P,FONS F,RAPIOR S.Biological and pharmacological activity of higher fungi:20-year retrospective analysis[J].Cryptogamie Mycol,2006,27(4):311-333.[9]LO H C H,WASSER S P.Medicinal mushrooms for glycemic control in diabetes mellitus:History,current status,future perspectives,and unsolved problems (review)[J].Int J Med Mushrooms,2011,13(5):401-426.[10]de SILVA D D,RAPIOR S,SUDARMAN E,et al.Bioactive metabolites from macrofungi:Ethnopharmacology,biological activities and chemistry[J].Fungal Divers,2013,62:1-40.[11]刘春如.香菇的分布概况及生物学特性[J].中国林副特产,2001(4):32-33.[12]陈忠纯.野生香菇人工栽培试验[J].浙江食用菌,1995(5):12.[13]潘迎捷,谭琦,陈明杰,等.香菇遗传研究和菌种选育的现状及发展[J].食用菌学报,1998(3):59-64.[14]王小军,武紫娟.凉山州林区野生菌资源保护性开发初探[J].特种经济动植物,2018,21(9):42-43.[15]WHITE T J,BRUNS T,LEE S,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenies[J].PCR Protocols:A Guide to Methods and Applications,1990,18(1):315-322.[16]黎倩,黄忠乾,李伟,等.两株野生侧耳属菌株鉴定及生物学特性研究[J].西南农业学报,2016,29(5):1209-1213.[17]宋冰,付永平,郭昱秀,等.一株野生侧耳属菌株的鉴定及生物学特性[J].北方园艺,2017(24):182-188.[18]李玉.图力古尔中国长白山蘑菇[M].北京:北京科学出版社,2003.[19]袁思明,房丽丽,何德,等.一株野生香菇的鉴定及菌丝最佳生长条件研究[J].湖北民族学院学报(自然科学版),2016,34(1):77-80.[20]HIBBETT D S,FUKUMASA-NAKAI Y,TSUNEDA A,et al.Phylogenetic diversity in shiitake inferred from nuclear ribosomal DNA sequences[J].Mycologia,1995,87(5):618-638.[21]SONG X X,ZHAO Y,SONG C Y,et al.ITS1/5.8 S/ITS2,a good marker for initial classification of shiitake culinary-medicinal Lentinus edodes (Agaricomycetes) strains in China[J].International Journal of Medicinal Mushrooms,2018,20(3):227-242.[22]SHARMA V P,KAMAL S,UPADHYAY R C,et al.Taxonomy,phylogeny,cultivation and biological activities of a Lentinus species from Andaman & Nicobar Islands (India)[J].Emirates Journal of Food and Agriculture,2015,27(8):570-576.[23]熊雪,李鹏,廖小锋,等.野生马桑香菇的鉴定及其生物学特性[J].北方园艺,2021(4):118-123.[24]佩格勒 D N,杨 T W K,姚一建.香菇的学名及其在当代真菌分类中的位置[J].真菌学报,1993(3):226-231.[25]张金霞.中国食用菌菌种学[M].北京:中国农业出版社,2011.[26]屈志松.香菇生长发育对环境条件的要求[J].现代农村科技,2011(11):15-16.[27]覃宝山,覃勇荣,黄春凤,等.香菇培养基主要营养物质筛选及培养条件的优化[J].中国园艺文摘,2017,33(8):1-4.[28]张建新,王增信,秦清军,等.秦香1号香菇最佳营养生长条件研究[J].西北农业学报,2002(2):97-100.[29]胥玲,朱莉,王璐,等.香菇L26的菌丝培养特性及系统发育学分析[J].种子,2013,32(6):5-8.[30]吴萍民.几种祁连山野生食用菌生物学特性及分类鉴定的研究[D].兰州:兰州交通大学,2014.

Memo

Memo:
-
Last Update: 2022-05-25