|Table of Contents|

Effects on Mycelial and Microstructural Characteristics of Repeated Subculture of Lentinula edodes

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年03
Page:
118-124
Research Field:
Publishing date:

Info

Title:
Effects on Mycelial and Microstructural Characteristics of Repeated Subculture of Lentinula edodes
Author(s):
LIU Na; SONG Ying; ZHANG Min; LYU Litao; ZHANG Shiyi; LI Yue
(Edible Fungi Research Institute,Liaoning Academy of Agricultural Sciences,Shenyang,Liaoning 110161)
Keywords:
Lentinula edodes; subculture; degeneration; micromorphology
PACS:
-
DOI:
10.11937/bfyy.20212982
Abstract:
The Lentinula edodes strain ‘Liaofu-4’ was used as test material,the effects of subculture times on mycelial growth characteristics and micromorphological changes were studied by subculture,in order to provide a reference for strain application and the study of the strain degradation in the process of subculture.The results showed that subculture times had a significant effect on the growth characteristics and micromorphology of mycelia,and the isolate parts of the fruiting body and the composition of culture medium affect the growth rate and state of the subcultured mycelia apparently.With the increase of subculture times,the growth rate of mycelia decreased significantly.After subcultured for 40 times,the growth rate of mycelia isolated from the pileus decreased by 35.62% on sawdust medium,and the mycelia isolated from the junction of the pileus and the stipe decreased by 34.05% on PDA,and the mycelial growth sparse.With the increase of subculture times,the number of mycelia branches and clamp-connection decreased gradually,and the width of mycelia clamp-connection isolated from pileus increased gradually.In conclusion,the subculture of L.edodes could cause the decrease of mycelial growth rate,the weakening of mycelial growth state,and the change of micromorphology including the width of mycelia,the width and quantity of clamp-connection.The study of mycelial characteristics showed isolation part from pileus and the junction of pileus and stalk were better choice.

References:

[1]杨瑞恒,吴莹莹,宋春艳,等.基于公共数据库信息对中国野生香菇种质资源地理分布的再分析[J].菌物学报,2018,37(3):335-351.[2]周峰,曹斌,尚晓冬,等.日本香菇菌种研发现状及生产品质管控特点[J].中国食用菌,2017,36(3):65-69,75.[3]安学明,陈超,刘小霞,等.草菇继代培养中菌种退化对子实体营养成分的影响[J].菌物学报,2020,39(2):390-397.[4]黄年来.食用菌百科[M].北京:农业出版社,1993.[5]陈士瑜.食用菌生产大全[M].北京:农业出版社,1988.[6]张功,候枝莲,峥嵘.三种菇子实体不同部位组织分离培养菌丝生长研究[J].中国食用菌,2001(2):9-10.[7]刘进杰,王淑芳,卜庆梅,等.四种食用菌子实体不同部位组织分离菌丝长势对比[J].烟台职业学院学报,2006(2):62-65.[8]王秀艳.子实体组织分离法制备食用菌母种[J].赤峰学院学报(自然科学版),2016,32(17):13-14.[9]程爽爽,张姣,杜双田.香菇单核菌株菌丝生长特性分析[J].中国食用菌,2019,38(7):32-37,42.[10]张清,张雅宁,罗金州.几个姬菇菌株的菌丝培养性状及出菇品比研究[J].四川农业科技,2019(7):46-50.[11]黄敏敏,陈宇航,姚清华.正红菇、牛肝菌外生菌根内层形态和结构的扫描电镜观察[J].热带作物学报,2013,34(11):2097-2101.[12]潘迎捷,汪昭月,龚胜萍,等.香菇菌种退化的遗传分析[J].食用菌,1991(2):12-13.[13]常远程,李彤.十二个香菇菌株培养性状观察比较[J].食用菌,2004(5):11-12.[14]蔡继炯,吴友吕,邵月香.香菇超微结构的电镜观察[J].食用菌,1983(4):31-33.[15]黄敏敏,刘洋,颜振兰,等.酸碱度对秀珍菇新品种福秀163菌丝体形态及锁状个数的影响[J].热带农业科学,2019,39(9):68-73.[16]黄敏敏,张闽春,黄晓丽,等.酸碱度对秀珍菇新品种福秀5669菌丝体形态及锁状个数的影响[J].食用菌,2019,41(4):15-18.[17]廖浩锋,周振辉,陈东梅,等.食用菌工厂化栽培中菌种扩繁的质量控制浅析[J].中国食用菌,2019,38(1):16-20.

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Last Update: 2022-05-04