|Table of Contents|

Isolation and Identification of Pathogen Causing Leaf Spot Disease in Nervilia fordii (Hance) Schltr. and Its Biocontrol

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年24
Page:
115-121
Research Field:
Publishing date:

Info

Title:
Isolation and Identification of Pathogen Causing Leaf Spot Disease in Nervilia fordii (Hance) Schltr. and Its Biocontrol
Author(s):
YANG Xinfeng1; 2; ZHOU Yaqin1; 3; TAN Xiaoming1; 2; YUAN Changge1; 2; FU Peng1; TANG Hongzhen1
(1.College of Pharmacy,Guangxi University of Chinese Medicine,Nanning,Guangxi 530200；2.Guangxi Engineering Technology Research Center of Advantage Chinese Patent Drug and Ethnic Drug Development,Nanning,Guangxi 530200;3.Guangxi Botanical Garden of Medicinal Plant,Nanning,Guangxi 530023)
Keywords:
Nervilia fordii (Hance) Schltr.; Cercospora canescens; endophytic fungi; antagonism
PACS:
-
DOI:
10.11937/bfyy.20212912
Abstract:
The leaves of wild Nervilia fordii (Hance) Schltr. infected with leaf spot disease were used as test materials.The pathogen of the leaf spot of N.fordii was obtained by tissue separation method,and identified by molecular and morphological methods.The pathogen was verified according to Koch′s postulates.Subsequently,the antagonistic effect of endophytic fungi isolated from N.fordii on the growth of the pathogen was detected by plate confrontation method and cross method,in order to explore the pathogen causing leaf spot of Nervilia fordii (Hance) Schltr.,and provide atheoretical basis for effective prevention and treatment of the disease.The results showed that C.canescens was identified as the pathogen of leaf spot in N.fordii reservoir.Then,8 kinds of endophytic fungi with obvious antagonistic effects were screened,with the inhibition rate of 44%.Among the eight strains,1151 (Penicillinum sp.) had the best antagonistic effect on the pathogen,up to 59.8%.

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Last Update: 2022-03-29