|Table of Contents|

Optimizing Culture Medium and Antioxidant Activity of Extracellular Polysaccharides in Liquid Fermentation of Stropharia rugosoannulata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年24
Page:
105-115
Research Field:
Publishing date:

Info

Title:
Optimizing Culture Medium and Antioxidant Activity of Extracellular Polysaccharides in Liquid Fermentation of Stropharia rugosoannulata
Author(s):
JIA Jiao1; 2; XIE Xiuchao1; 2; SONG Yu1; 2; SUN Ya1; 2; XU Yijie1; 2; YAO Song1
(1.School of Biological Science and Engineering,Shaanxi University of Technology,Hanzhong,Shaanxi 723000;2.Shaanxi Provincial Engineering Research Edible Medicinal Microbes,Hanzhong,Shaanxi 723000)
Keywords:
Stropharia rugosoannulata; extracellular polysaccharides; response surface methodology; medium optimization; antioxidant
PACS:
-
DOI:
10.11937/bfyy.20212781
Abstract:
The liquid fermentation conditions for Stropharia rugosoannulata D5 liquid medium in shake flasks were optimized by single factor experiment combined with response surface method,and the antioxidant activity of extracelluar polysaccharides was studied,in order to provide a reference for the production of liquid inoculum and the development of extracellular polysaccharides.The results showed that the optimal liquid fermentation conditions were glucose 30.3 g·L-1,yeast extract 3.7 g·L-1,peptone 1.0 g·L-1,KH2PO42.0 g·L-1,MgSO4·7H2O 1.0 g·L-1 and vitamin B1 19.0 mg·L-1.The yield of mycelia and extracellular polysaccharides under this fermentation process was 1.317 g·(200mL)-1 and 230.09 mg·L-1,respectively,which were 37.7% and 15.9% higher than those before optimization.Extracellular polysaccharides was obtained from the fermentation exhibited a strong scavenging effect on Hydroxyl,DPPH and ABTS+ free radicals,and the scavenging rate increased with the increase of concentration.

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Last Update: 2022-03-28