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Effects of Bacillus subtilis and Trichoderma harzianum on the Growth of Cucumber Seedlings

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年23
Page:
11-20
Research Field:
Publishing date:

Info

Title:
Effects of Bacillus subtilis and Trichoderma harzianum on the Growth of Cucumber Seedlings
Author(s):
ZHANG Zuxian1; 2; DENG Wei1; 2; LI Chun1; 2; XU Hongwei1; 2; ZHOU Xiaofu1; 2
(1.Collage of Life Science,Jilin Normal University,Siping,Jilin 136000；2.Jilin Provincial Key Laboratory of Plant Resources Science and Green Production,Jilin Normal University,Siping,Jilin 136000)
Keywords:
Bacillus subtilis; Trichoderma harzianum; Cucumis sativus L.; root system
PACS:
-
DOI:
10.11937/bfyy.20212546
Abstract:
Cucumis sativus L.‘Jinyan No4’ was used as experimental material,a pot experiment was adopted.LB medium (100% root irrigation,CK1),Bacillus subtilis (100% root irrigation,TK1),PD medium (100% root irrigation,CK2),Trichoderma harzianum (100% root irrigation,TK2),mixed medium (50% LB medium+50% PD medium,CK3) and mixed bacterial agents were set (50% Bacillus subtilis+50% Trichoderma harzianum root irrigation,TK3),the root morphology was studied by analyzing the fresh quality of seedling plants and root cap characters,the root distribution and root configuration of cucumber were studied by WinRhizo root scanner,the correlation between the geometric characteristics of root configuration was obtained by factor analysis and Pearson correlation analysis,the principal component score and comprehensive score were calculated by principal component analysis,and the different bacterial agents were compared by cluster analysis.The effects of Bacillus subtilis and Trichoderma harzianum on plant roots were studied,in order to provide a reference for the rational application of microbial agents.The results showed that different bacterial agents had no significant effects on the specific root length,root tissue density,root fineness,branch density and fractal dimension of cucumber seedlings (P>005).Under the condition of Bacillus subtilis,the root surface area and root tissue density of cucumber seedlings increased the most,and the specific root length and specific root surface area decreased the most;the application of Trichoderma harzianum increased the root length,root volume and link number of cucumber roots the most,and the branch density decreased the most;the application of mixed bacterial agent affected the aboveground dry mass,underground dry mass,root shoot ratio,branch number and crossing number of cucumber seedlings.The correlation analysis showed that the main influencing factors of root configuration were link number,root surface area and root volume.The comprehensive scores of cucumber roots treated with different bacterial agents were as follows,mixed bacterial agent>Bacillussubtilis>Trichoderma harzianum>PD medium>LB medium>mixed medium.The comprehensive evaluation value of length and configuration was high,the root evaluation was medium under the treatment of PD medium,LB medium, Bacillus subtilis and Trichoderma harzianum,and the root evaluation was the lowest under the treatment of mixed medium.

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Last Update: 2022-02-21