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Endogenous Hormone Changes and Related Genes Expression Analysis in the Formation of Leafy Head of Tetraploid Chinese Cabbage

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年01
Page:
1-8
Research Field:
Publishing date:

Info

Title:
Endogenous Hormone Changes and Related Genes Expression Analysis in the Formation of Leafy Head of Tetraploid Chinese Cabbage
Author(s):
MENG Chuan; LIU Xiaodong; WU Fang; WANG Yuhai; MU Jingui; WANG Mingqiu
(Institute of Cash Crops,Hebei Academy of Agricultural and Forestry Sciences/Hebei Vegetable Engineering Technology Centre,Shijiazhuang,Hebei 050051)
Keywords:
tetraploid Chinese cabbage; leaf ball; gene function; qRT-PCR; endogenous hormone
PACS:
-
DOI:
10.11937/bfyy.2021251
Abstract:
The tetraploid cabbage of overlopping type and outward-cyrling type were used as test materials.Firstly,the expression of the mid abscisic acid-related gene Bra018800 and the growth hormone-related gene Bra018750 was analysed by quantitative real-time fluorescence (qRT-PCR).Subsequently,the endogenous hormones were measured by using an enzyme-linked immunoassay,including the resolution of endogenous hormone changes and related gene expression changes in tetraploid cabbage leaf bulb formation,in order to provide a reference for subsequent studies on the mechanism of leaf bulb formation in different leaf bulb types of tetraploid cabbage.The results showed that the expression of Bra018800 and Bra018750 genes was the highest in the heart-wrapping and rosette stages of tetraploid cabbage of overlopping type,with 2.56-2.80-fold and 5.18-6.59-fold higher expression than the same period in the outward-cyrling type,respectively.The results of the endogenous hormone assay showed that the two hormones ABA and IAA were significantly different between the two types of tetraploid cabbage.The highest levels of ABA were found in the heart-wrapping stage of the tetraploid cabbage of overlopping type,which was 2.05-2.12-fold higher than that of the heart-wrapping stage of the tetraploid cabbage of outward-cyrling,consistent with the expression trend of the abscisic acid-related gene 〖STBX〗Bra018800 〖STBZ〗in both types of material.IAA content at the rosette stage of tetraploid cabbage of overlopping type was significantly higher than that at the same period of the outward-cyrling type by 2.58-2.60-fold,consistent with the expression trend of the growth hormone-related gene Bra018750 in both types of materials.In conclusion,Bra018750 and Bra018800 genes regulate the synthesis of endogenous hormones IAA and ABA and play a key role in the formation of leaf bulb types during the heart-wrapping and rosette stages of tetraploid cabbage,respectively.

References:

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Last Update: 2022-03-30