|Table of Contents|

Cloning,Bioinformatics and Expression Analysis of ERFb2 Transcription Factor in Tomato

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年23
Page:
1-10
Research Field:
Publishing date:

Info

Title:
Cloning,Bioinformatics and Expression Analysis of ERFb2 Transcription Factor in Tomato
Author(s):
JIN Baoxia123WEI Xiaohong24ZHU Xiaolin234WANG Weijie123
(1.College of Life Science and Technology,Gansu Agricultural University,Lanzhou,Gansu 730070;2.Gansu Key Lab of Crop Genetic & Germplasm Enhancement,Lanzhou,Gansu 730070;3.Gansu Provincial Key Lab of Aridland Crop Science,Lanzhou,Gansu 730070;4.College of Agronomy,Gansu Agricultural University,Lanzhou,Gansu 730070)
Keywords:
tomatoERFb2bioinformaticsexpression analysis
PACS:
-
DOI:
10.11937/bfyy.20212435
Abstract:
The SlERFb2 gene of tomato was cloned,and the structure of the encoded protein was analyzed by using bioinformatics methods,and the expression level of the SlERFb2 was detected by qRT-PCR in different treatments and different periods, in order to provide some theoretical basis for the response mechanism of tomato to abiotic stress.The results showed that the SlERFb2 contained 5 openreading frames,encoding a total of 258 amino acids,which was an acidic protein;its secondary structure was mainly α-helix,and the predicted protein was located on the nucleus;and contained 39 phosphorylationsites with 15 glycosylation sites;the promoter region was mainly I-box,and contained multiple homeopathic elements that induce gene expression;the tertiary structure was relatively simple,and the transcription factor had 4 interactions in tomato protein,phylogenetic tree,the closest relationship between tomato and potato;qRT-PCR showed that the gene was particularly sensitive to low temperature response.

References:

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Last Update: 2022-02-21