|Table of Contents|

Optimization for the Medium for Liquid Spawn and Culture Conditions of Dictyophora rubrovolvata

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年22
Page:
124-129
Research Field:
Publishing date:

Info

Title:
Optimization for the Medium for Liquid Spawn and Culture Conditions of Dictyophora rubrovolvata
Author(s):
LU Zhenghua1; 2; 3; WEN Tingchi2; 3; XU Yanjun3; YU Gangjiang1; 2; 3; YANG Yu1; 2; 3
(1.College of Liquor and Food Engineering,Guizhou University,Guiyang,Guizhou 550025;2.The Engineering Research Center of Southwest Bio-Pharmaceutical Resources,Ministry of Education,Guizhou University,Guiyang,Guizhou 550025;3.Mushroom Research Institute,Guizhou University,Guiyang,Guizhou 550025)
Keywords:
Dictyophora rubrovolvata; liquid strains; shaking flaskculture; culture medium optimization
PACS:
-
DOI:
10.11937/bfyy.20212273
Abstract:
Taking Dictyophora rubrovolvata as test material,the carbon and nitrogen source selection and culture condition optimization of its liquid medium of Dictyophora rubrovolvata were studied through liquid shake flask culture method,in order to provide a reference for the preparation and extended culture of liquid strains of Dictyophora rubrovolvata.The results showed that glucose and peptone were the optimal carbon source and nitrogen source,respectively.The optimal formula was 30 g?L-1 of glucose,1.5 g?L-1of peptone,2 g?L-1of KH2PO4,1 g?L-1of MgSO4,1 g?L-1 of (NH4)2SO4,0.016 g?L-1 of vitamin C,and 0.0016 g?L-1 of indole acetic acid (IAA).Besides,the optimal culture conditions were 25 ℃,150 r?min-1 of shaking speed,and 20 days of culture period.The optimal culture conditions were as follows 500 mL of liquid per 1 000 mL of culture volume,15% of inoculation size,and 15 days of culture period;35 days were required from first-degree species preparation to the extended culture.

References:

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Last Update: 2022-02-18