|Table of Contents|

Response of Nutrient Content and Key Enzyme Activity of Nitrogen Metabolism to Nitrogen Level in Walnut Seedling

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2022年01
Page:
40-46
Research Field:
Publishing date:

Info

Title:
Response of Nutrient Content and Key Enzyme Activity of Nitrogen Metabolism to Nitrogen Level in Walnut Seedling
Author(s):
SONG Yan1; 2; 3; GAO Shan2; WANG Xiaohai1; 3; 4; ZHANG Rui1; 2; 3; Mutarifu·AIHETI2; 3
(1.Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basil,Xinjiang Production and Construction Corps,Alar,Xinjiang 843300;2.College of Plant Science and Technology,Tarim University,Alar,Xinjiang 843300;3.National and Local Joint Engineering Laboratory of High Efficiency and Superior-quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Trees in South,Alar,Xinjiang 843300;4.College of Life Science,Tarim University,Alar,Xinjiang 843300)
Keywords:
walnut seedling; grow; nitrogen metabolizing enzyme; nutrient elements
PACS:
-
DOI:
10.11937/bfyy.20212186
Abstract:
Taking one-year-old walnut seedlings as the experimental materials,different nitrogen treatments were set as N1:5 g·plant-1,N2:10 g·plant-1,N3:15 g·plant-1 and N4:20 g·plant-1,respectively,and water treatment was used as control.The plant height,ground diameter,leaf nutrient content and key enzyme activities of nitrogen metabolism of walnut seedlings were studied,in order to provide a reference for the formulation of scientific fertilization strategy and efficient nutrient management in the production of walnut seedlings.The results showed that the plant height and ground diameter of walnut seedlings in different nitrogen levels tended to be lifted first and then reduced,and the plant height and ground diameter of walnut seedlings in Aksu provenance and Hotan provenance reached the peak value in N2 and N3,respectively.The protein content and nitrate reductase activity of walnut seedlings under different treatments showed a trend of lifting first and then inhibition,and reached the peak value after N2 treatment.The protein content and nitrate reductase activity of walnut seedlings were (A:26.242 7 mg·g-1,H:25.436 0 mg·g-1) and (A：34.982 4 μg·g-1·h-1,H：34.199 0 μg·g-1·h-1),respectively,which were significantly higher than N0.At the same time,nitrogen promoted the increase of glutamine synthase activity.Nitrogen promoted the accumulation of elements N,P and K in the leaves of walnut seedlings from both provenances,and the accumulation of elements P was inhibited after the nitrogen application amount exceeded N3 treatment,which was significantly higher than that of the control group (1.31 times and 2.00 times,respectively).Therefore,nitrogen played a key role in the growth and development of walnut seedlings,and appropriate nitrogen application rate can promote the growth of walnut seedlings,nitrogen assimilation and nutrient content accumulation.

References:

[1]SOLOMONSON L P,BARBER M J.Assimilatory nitrate reductase:Functional properties and regulation[J].Ann Rev Plant Physiol,1990,41(1):2569-2579.[2]王小纯,王晓航,熊淑萍,等.不同供氮水平下小麦品种的氮效率差异及其氮代谢特征[J].中国农业科学,2015,48(13):2569-2579.[3]刘连涛,孙红春,张永江,等.不同氮营养水平对转基因棉花叶片衰老生理特征及微量元素含量的影响[J].河北农业科学,2011,15(7):90.[4]徐照丽,邓小鹏,杨宇虹,等.氮水平和硝态氮比例对烤烟钙、镁、铜、锌等元素累积分配的影响[J].基因组学与应用生物学,2018,37(2):900-908.[5]吴国良,刘群龙,郑先波,等.核桃种质资源研究进展[J].果树学报,2009,26(4):539-545.[6]李忠新,杨莉玲,阿布力孜·巴斯提,等.中国核桃产业发展研究[J].中国农机化学报,2013,34(4):23-28.[7]李忠新,杨莉玲,阿布力孜·巴斯提,等.新疆核桃产业化发展研究[J].新疆农业科学,2014,51(5):973-980.[8]李源,马文强,朱占江,等.新疆核桃产业发展现状及对策建议[J].农学学报,2019,9(7):80-86.[9]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.[10]孔祥生,易现峰.植物生理学实验技术[M].北京:中国农业出版社,2008.[11]MA J Z,ZHANG M,LIU Z G,et al.Effects of foliar application of the mixture of copper and chelated iron on the yield,quality,photosynthesis,and microelement concentration of table grape (Vitis vinifera L.)[J].Scientia Horticulturae,2019,254(1):106-115.[12]MERGHANY M M.Effect of irrigation system and nitrogen levels on vegetative growth,yield components and some chemical composition of tomato plants growth in newly reclaimed sandy soils[J].Annals of Agricultural Science,1997,35(2):965-981.[13]刘根华,黄坚钦,潘春霞,等.基于反射光谱的山核桃幼苗氮素营养状况分析[J].林业科学,2011,47(1):165-171.[14]康晓育,孙协平,常聪,等.氮素形态对不同苹果砧木幼苗生长的影响[J].西北农林科技大学学报(自然科学版),2013,41(6):133-138.[15]甘娜,陈其兵.施氮对天彭牡丹(红丹兰、丹景红)形态特征的影响[J].广东农业科学,2012,39(23):53-56.[16]王益明,万福绪,李瑞瑞,等.指数施肥对美国山核桃幼苗生长及养分积累的影响[J].东北林业大学学报,2018,46(9):21-25.[17]张会绒.不同施氮水平对小麦幼苗性状的影响[J].陇东学院学报,2010,21(5):77-80.[18]刘卫群,韩锦峰,史宏志,等.数种烤烟品种中碳氮代谢与酶活性的研究[J].中国农业大学学报,1998(1):22-26.[19]李明,梁晓红,熊德中.不同肥力植烟土壤上施氮水平对烤烟氮代谢的影响[J].热带作物学报,2014,35(2):271-276.[20]赵春波,王超楠,宋述尧,等.氮素营养水平对黄瓜氮代谢关键酶活性变化及氮化物的影响[J].吉林农业大学学报,2017,39(2):139-147.[21]赵艺欣,阚宏江,刘玉莲,等.不同硝态氮肥用量对春小麦叶片氮代谢的影响[J].江苏农业科学,2012,40(3):55-57.[22]朱玉珍,惠竹梅,张辉.不同施氮水平对‘赤霞珠’葡萄叶片氮素化合物及关键酶活性的影响[J].北方园艺,2012(16):142-145.[23]祝燕,刘勇,李国雷,等.氮素营养对长白落叶松移植苗生长及养分状况的影响[J].林业科学,2011,47(9):168-172.[24]王益明,卢艺,张慧,等.指数施肥对美国山核桃幼苗生长及叶片养分含量的影响[J].中国土壤与肥料,2018(6):136-140.[25]倪玉琼,张强,曹方琴,等.不同施氮量对高粱产量及植株养分积累的影响[J].水土保持研究,2016,23(5):95-99,105.[26]张刚,翁悦,李德香,等.铜胁迫对黑麦草种子萌发及幼苗生理生态的影响[J].东北师大学报(自然科学版),2019,51(1):119-124.[27]秦世玉,孙学成,胡承孝,等.钼肥对甘蓝型油菜薹期碳氮代谢的影响[J].华北农学报,2016,31(4):227-232.[28]NIE Z J,HU C X,LIU H E,et al.Differential expression of molybdenum transport and assimilation genes between two winter wheat cultivars (Triticum aestivum)[J].Plant Physiology & Biochemistry,2014,82(1):27-33.

Memo

Memo:
-
Last Update: 2022-04-01