|Table of Contents|

Effects of Low Temperature Acclimation on Endogenous Polyamine Content and Expression of Biosynthesis and Decomposition Genes in Phaseolus vulgaris L.

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年23
Page:
30-37
Research Field:
Publishing date:

Info

Title:
Effects of Low Temperature Acclimation on Endogenous Polyamine Content and Expression of Biosynthesis and Decomposition Genes in Phaseolus vulgaris L.
Author(s):
LIU Chang; LI Jiayin; FENG Guojun; LIU Dajun; YAN Zhishan; YANG Xiaoxu
(College of Modern Agriculture and Ecological Environment,Heilongjiang University,Harbin,Heilongjiang 150030)
Keywords:
snap bean; polyamines; low temperature acclimation; gene expression
PACS:
-
DOI:
10.11937/bfyy.20212160
Abstract:
Seven snap beanvarieties were used as test materials.Cold injury index and relative electrolyte permeability were used to screen low temperature tolerant and low temperature sensitive snap bean varieties,and the content of endogenous polyamine in the process of low temperature acclimation was determined to study the changes of putrescine,spermidine and spermine content in response to low temperature acclimation,as well as the effects on gene expression of polyamine synthesis and decomposing enzymes,in order to provide reference for the application of polyamines to alleviate low temperature stress in snap bean in the future.The results showed that the contents of putrescine and spermidine in the leaves of the two cultivars increased significantly during the low temperature acclimation,but the change of spermidine content was not obvious.In the process of low temperature acclimation,the expression of polyamine synthase gene ADC increased,the expression of ODC did not change significantly,the expression of SAMDC increased,SPDS and SPMS were inconsistent in the two varieties;the expression of polyamine lyase gene DAO and PAO increased,SMO was inconsistent in the two varieties.In conclusion,the accumulation of putrescine and spermidine in plants may be related to the improvement of low temperature tolerance of Snap bean seedlings,and their accumulation may be related to the expression levels of ADC,SAMDC,DAO and PAO.

References:

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Last Update: 2022-03-10