|Table of Contents|

Embryo Rescue Technique Create New Seedless，Cold-resistant Grapevines Germplasm

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年22
Page:
29-37
Research Field:
Publishing date:

Info

Title:
Embryo Rescue Technique Create New Seedless，Cold-resistant Grapevines Germplasm
Author(s):
YU Saisai1; 2; 3; LI Shasha1; 2; 3; LUO Qiangwei4; XU Yan1; 2; 3; WANG Yuejin1; 2; 3
(1.College of Horticulture,Northwest Agriculture and Forestry University,Yangling,Shaanxi 712100；2.State Key Laboratory of Crop Stress Biology in Arid Areas,Yangling,Shaanxi 712100；3.Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest,Ministry of Agriculture and Rural Affairs,Yangling,Shaanxi 712100；4.Institute of Grapes and Melons on the Research in Xinjiang,Shanshan,Xinjiang 838200)
Keywords:
grapevine; embryo rescue; seedlessness; cold resistance; molecular marker
PACS:
-
DOI:
10.11937/bfyy.20212010
Abstract:
The European seedless grape varieties and the progeny of cold-hardy Chinese wild Vitis amurensis crossed with ‘Zuoyouhong’ and ‘Beibinghong’，as well as other cold resistant materials were used as female and male parents to hybrid in the field.Immature berries were gathered before embryo abortion，and hybrid progenies were obtained in vitro embryo rescue.Additionally，the molecular markers were applied to screen the new seedless and cold resistance grape from hybrid seedling.To improve the efficiency of breeding seedless and cold resistance grapes by embryo rescue，the factors of hybrid ovules sampling time，parental genotype，different kinds and concentrations of amino acids in embryo germination medium were studied,in order to improve embryo rescue efficiency of cold-resistant grapevine.The results showed that 235 hybrid plants were obtained.The hybridization combination of ‘Ruby Seedless’ and ‘Kunxiang Seedless’ as the parent had better embryo rescue efficiency and were more suitable as the parent in seedless grape embryo rescue.It was better for embryo rescue efficiency to add glutamine on MM3 at the isolated developmental stage.There were 32 hybrids carried specific bands detected by seedless molecular marker SCF27-2000，38 hybrids carried specific bands detected by cold resistant molecular markers S241-717 and S238-800.

References:

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Last Update: 2022-02-11