|Table of Contents|

Conditions Optimization of Fallopia multiflora Seed Germination and Its Seedling Emergence

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年23
Page:
114-120
Research Field:
Publishing date:

Info

Title:
Conditions Optimization of Fallopia multiflora Seed Germination and Its Seedling Emergence
Author(s):
LI Juan; LIN Haidan; TONG Jiayun
(School of Pharmaceutical Sciences,Guangzhou University of Chinese Medicine,Guangzhou,Guangdong 510006)
Keywords:
Fallopia multiflora; seed germination; GA3; seedling emergence
PACS:
-
DOI:
10.11937/bfyy.20211929
Abstract:
Taking Fallopia multiflora seeds as test materials,four temperature groups (20-25 ℃,20-30 ℃,25 ℃,and 25-30 ℃) and two germination beds groups (top of paper and top of sand) were set up.Three concentrations of gibberellin acid (GA3) solution (0125,0250,0500 mg·mL-1,distilled water as the control) were used to presoak the seeds for 24 hours before sowing.In the seedling emergence test,effects of three kinds of culture mediums (pond sludge,sand,mixture of pond sludge with sand) and sowing depths (0 cm,05 cm and 10 cm) were investigated.Parameters of germination percentage (GP),germination energy (GE),germination index (GI),fresh weight of seedlings,vigor index (VI),and lengths of radicals and seedlings were used to evaluate the effects,in order to provide a reference for the seed quality testing and seedling cultivation of Fallopia multiflora.The results showed that seeds cultured at 20-25 ℃ (night/day) acquired the highest GP (758%).Seeds cultured at constant 25 ℃ (on the top of paper) acquired the highest GE.Compared to the control,the GPs of all the groups presoaked by GA3 solutions were increased significantly.The highest GP was 870%,while the concentration was 0500 mg·mL-1.Seeds sown in pond sludge acquired the highest seedling emergence.Seedlings cultured in sands possessed the longest radicles.Seeds sown in pond sludge at 05 cm depth acquired the highest germination energy.However,seedling emergence was significantly inhibited while the depth increased to 10 cm.

References:

[1]国家药典委员会.中华人民共和国药典：一部[S].北京:中国医药科技出版社,2020.[2]LI A R,BAO B J,GRABOVSKAYA-BORODINA A E,et al.Flora of China[M].Beijing:Science Press,2003.[3]吴庆华,董青松.何首乌人工栽培研究进展[C].南宁:2009年中国植物园学术年会暨广西药用植物园50岁华诞,2009.[4]胡诚,齐迎春,余展琛,等.何首乌块根栽培试验[J].中药材,1997,20(11):545-546.[5]唐春梓,刘海华,艾伦强,等.何首乌栽培资源调查[J].宁夏农林科技,2013,54(1):14-15.[6]曾文丹,陆柳英,谢向誉,等.何首乌繁殖技术研究进展[J].中国热带农业,2016,13(3):66-68.[7]张萃蓉,潘世民,曾维群,等.何首乌栽培试验[J].中药材,1997,20(5):217-218.[8]张鲁文,程炳高,邹琦.何首乌的组织培养[J].植物生理学通讯,1987,23(1):28.[9]衷维纲,刘素珍.何首乌茎切段培养[J].中草药,1987,18(2):29-31.[10]李娟玲,刘国民,邱文华,等.何首乌茎段离体培养的研究[J].贵州科学,2003,21(3):86-91.[11]HUANG H P,WANG J,HUANG L Q.Germplasm preservation in vitro of Polygonum multiflorum Thunb[J].Pharmacognosy Magazine,2014,10(38):179-184.[12]KANG Y,LEE K,CHOI J,et al.Maximizing seedling and root tuber production in Polygonum multiflorum for use in ethnomedicine[J].South African Journal of Botany,2018,119:119-131.[13]KIM Y G,KOMAKECH R,JEONG D H,et al.Verification of the field productivity and bioequivalence of a medicinal Plant (Polygonum multiflorum) developed using an in vitro culture method[J].Plants,2020(9):1280.[14]王华磊,徐绯,赵致,等.何首乌种子发芽试验研究[J].农学学报,2012,2(1):1-3.[15]刘丽娜,关文灵.何首乌种子萌发对温度、光照和外源生长调节物质的响应[J].中药材,2012,35(11):1732-1735.[16]杨洋,张丽慧,张玲燕.何首乌种子发芽特性研究[J].河南农业,2016,27(7):36-37,41.[17]肖承鸿,周涛,陈敏,等.何首乌种子品质检验及质量分级标准研究[J].时珍国医国药,2015,26(8):2017-2021.[18]中华人民共和国国家标准GB/T 35434-1995.农作物种子检验规程[S].北京:中国标准出版社,1995.[19]支巨振.《农作物种子检验规程》实施指南[M]北京:中国标准出版社,2000.

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Last Update: 2022-03-16