|Table of Contents|

Establishment of Regeneration System and Genetic Transformation System of ‘Yingjie Hongfei’ of Chrysanthemum morifolium

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年21
Page:
72-77
Research Field:
Publishing date:

Info

Title:
Establishment of Regeneration System and Genetic Transformation System of ‘Yingjie Hongfei’ of Chrysanthemum morifolium
Author(s):
QUAN Yingjie; REN Manrong; YANG Wenting; ZHANG Jianing; HE Zihan; GAO Ri
(Agricultural College，Yanbian University，Yanji，Jilin 133002)
Keywords:
Chrysanthemum morifolium; regeneration system; adventitious bud; carboxybenzylpenicillin; hygromycin
PACS:
-
DOI:
10.11937/bfyy.20211861
Abstract:
Chrysanthemum morifolium ‘Yingjie Hongfei’ were used as test material.Tissue culture method was carried out.The effects of 6-BA,NAA concentration,sucrose concentration,MS concentration,NO-3 and NH+4 concentration ratio,Carb concentration and Hyg concentration on adventitious bud differentiation of test tube plantlets of Chrysanthemum morifolium ‘Yingjie Hongfei’ were studied,in order to establish an efficient regeneration system of ‘Yingjie Hongfei’ and lay a foundation for transgenic system of Chrysanthemum morifolium.The results showed that the callus induction and adventitious bud differentiation rate of ‘Yingjie Hongfei’ leaves reached the highest in 3/4MS medium with 6-BA and NAA concentration of 2.0 mg·L-1 and 0.6 mg·L-1,sucrose concentration of 30 g·L-1,NO-3∶NH+4 concentration of 30∶30,and the average adventitious bud differentiation rate of each leaf disc was 3.22,with 75.67%.When 0.75 mg·L-1 IBA was added into rooting medium,root growth was the best.The concentration of Carb was 250 mg·L-1 and Hyg was 2.0 mg·L-1,which was suitable for selection.

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Last Update: 2022-01-14