|Table of Contents|

Establishment of Tissue Culture and Rapid Propagation System in Fraxinus velutina

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年19
Page:
78-83
Research Field:
Publishing date:

Info

Title:
Establishment of Tissue Culture and Rapid Propagation System in Fraxinus velutina
Author(s):
XU Dingfan; LIU Yanjun; WANG Zhaonan; HUANG Junxuan; WU Chunxia; LI Jianke
(College of Horticulture and Landscape，Tianjin Agricultural University，Tianjin 300392)
Keywords:
Fraxinus velutina; stem segment; tissue culture; phytohormone; medium
PACS:
-
DOI:
10.11937/bfyy.20211382
Abstract:
Taking the stem segment of Fraxinus velutina with axillary buds as test material,using tissue culture method,and the tissue culture seedlings were acclimated and transplanted.The effects of different disinfection time on explants,different types and concentrations of plant hormones on axillary bud proliferation,strong seedling culture and rooting culture were studied,in order to establish a perfect tissue culture rapid propagation system of Fraxinus velutina,and provide a reference for fine clone breeding and industrialized production of Fraxinus velutina.The results showed that,the optimal explant disinfection treatment was 70% alcohol disinfection for 30 seconds+2% sodium hypochlorite disinfection for 10 minutes，the pollution rate was only 4.44%，the highest survival rate was 90.00%，the mortality rate was 5.56%；the optimal proliferation medium was MS+0.5 mg?L-1 BA+1.0 mg?L-1 IAA，the proliferation coefficient was 5.52；the optimal seedling culture medium was MS+0.5 mg?L-1 IAA，the average seedling height was 5.83 cm；the optimal rooting medium was 1/2MS+0.5 mg?L-1 NAA，the rooting rate was 98.89%，the average number of roots per plant was 5.62；after rooting，the seedlings were domesticated under natural light for one week，and transplanted into peat∶vermiculite∶perlite=1∶1∶1 matrix，the survival rate was more than 90%.

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Last Update: 2022-01-04