|Table of Contents|

Microscopic and Molecular Identification of Bupleurum chinense Seeds From Different Areas

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年23
Page:
121-127
Research Field:
Publishing date:

Info

Title:
Microscopic and Molecular Identification of Bupleurum chinense Seeds From Different Areas
Author(s):
HAN Xiaowei1; FENG Jiaxing2; JIN Aihong2; LU Ruike2; LIU Lingdi2
(1.Traditional Chinese Medicine Processing Technology Innovation Center of Hebei Province,Hebei University of Chinese Medicine,Shijiazhuang,Hebei 050200;2.Institute of Cash Crops,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang,Hebei 050051)
Keywords:
Bupleurum chinense; seed; morphological characteristics; ITS2; DNA barcoding
PACS:
-
DOI:
10.11937/bfyy.20211253
Abstract:
Taking 28 Bupleurum chinense seeds as test materials,the genetic relationship of 28 Bupleurum chinense seeds was studied by the combination of seed appearance identification and DNA molecular identification,in order to provide reference for identifying Bupleurum chinense seeds by DNA barcoding combined with seed morphological characteristics.The results showed that with ITS2 analysis the seed sequences of Bupleurum chinense,Bupleurum smithii were clustered into one branch separately,B.longicaule var.amplexicaule and B.marginatum var.stenophyllum were clustered into one branch,which had good monophyly.The morphological characteristics of Bupleurum chinense seeds were consistent with ITS2.The research provided references for identifing Bupleurum chinense seeds by DNA barcoding combined with seed morphological characteristics.

References:

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Last Update: 2022-03-16