|Table of Contents|

Effects of Polyamines on Root Damage and Nitric Oxide Production in Malus hupehensis var.pingyiensis Under Cadmium Stress

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年21
Page:
31-38
Research Field:
Publishing date:

Info

Title:
Effects of Polyamines on Root Damage and Nitric Oxide Production in Malus hupehensis var.pingyiensis Under Cadmium Stress
Author(s):
JIANG Qianqian1YANG Hongqiang2
(1.Key Laboratory of Biochemistry & Molecular Biology in Universities of Shandong,Weifang University,Weifang,Shandong 261061;2.College of Horticulture Science and Engineering,Shandong Agricultural University/State Key Laboratory of Crop Biology,Tai′an,Shandong 271018)
Keywords:
Malus hupehensis var pingyiensisrootcadmium stresspolyaminesnitric oxide
PACS:
-
DOI:
10.11937/bfyy.20211026
Abstract:
Taking one-year-old Malus hupehensis Rehd.seedlings grown in nutrient solution as test materials,the endogenous free polyamines content,polyamine oxidase (PAO) activity,hydrogen peroxide (H2O2) content,cell damage,and mitochondria characteristics were studied after 48 hours by applying cadmium sulfate,spermine,and spermidine to the nutrient solution.In addition,seedlings were treated with polyamine synthesis inhibitors,and then the changes of nitric oxide (NO) production,nitric oxide synthase (NOS) activities,and nitrate reductase (NR) activities were measured,in order to reveal the physiological mechanism of exogenous spermine and spermidine in alleviating cadmium stress of Malus hupehensis Rehd.var.pinyiensis Jiang,and to explore the regulation of polyamines (PAs) on nitric oxide (NO) metabolism.The results showed that the H2O2 content was increased significantly,while the mitochondrial membrane permeability was increased,the membrane potential and cytochrome c/a were decreased,the NO production was increased and eventually root cells were severely damaged under cadmium stress.The metabolism of polyamines was abnormal,the spermine (Spm) and spermidine (Spd) contents were significantly reduced,the putrescine (Put) content was significantly increased,the (Spd+Spm)/Put ratio was significantly reduced,and the PAO activity was significantly increased.The PAO activity,H2O2 content,NOS activity,NR activity,and NO production were reduced by exogenous application of Spm and Spd under cadmium stress,which stabilized mitochondrial membrane permeability,increased membrane potential and cytochrome c/a,rescored (Spd+Spm)/Put ratio.The NOS,NR activity and NO production were increased significantly by exogenous application of polyamine synthesis inhibitors under cadmium stress.The above results showed that the exogenous addition of spermine or spermidine can maintain the stable function of root mitochondria by inhibiting the production of NO,obviously reducing the damage of root cells under cadmium stress,and improving the cadmium tolerance of plants.

References:

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Last Update: 2022-01-14