|Table of Contents|

Optimization of Extraction Process and Antioxidant Activity of Total Flavonoids From Platycodonis radix

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年20
Page:
104-110
Research Field:
Publishing date:

Info

Title:
Optimization of Extraction Process and Antioxidant Activity of Total Flavonoids From Platycodonis radix
Author(s):
SUN Xiaochun; HUANG Wenjing; REN Fei
(Co-construction Collaborative Innovation Center for Chineses Medicine Resources Industrialization by Shaanxi & Education Ministry，Shaanxi University of Chinese Medicine/State Key Laboratory of Research & Development of Characteristic Qin Medicine Resources （Cultivation）,Xianyang,Shaanxi 712083)
Keywords:
Platycodonis radix; total flavonoids; Plackett-Burman design; central composite design; antioxidant activity
PACS:
-
DOI:
10.11937/bfyy.20210808
Abstract:
The total flavonoids from Platycodonis radix was selected as research object.Plackett-Burman design and central composite design was used to optimize the extraction process,antioxidant activities were evaluated by three in vitro antioxidant assay,in order to provide a reference for the development and application of flavonoids from Platycodonis radix.The results showed that optimal extraction parameters were ethanol concentration 63%,liquid-solid ratio 22∶1 mL?g-1,soak time 63 minutes and ultrasonic time 10 minutes.Under these conditions,the extraction yield was 0.45%and the purity was 58.22%.The IC50 value of total flavonoids from Platycodonis radix and vitamin C for DPPH?was 0.546 mg?mL-1 and 8 μg?mL-1 respectively,for ?OH was 0.196 mg?mL-1and 0.368 mg?mL-1 respectively,total antioxidant capacity of Platycodonis radix was not significantly different from vitamin C.

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Last Update: 2022-02-10