|Table of Contents|

Cloning and Expression Analysis of an Auxin Response Factor Gene in Cerasus pseudocerasus ‘Manaohong’

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年19
Page:
7-14
Research Field:
Publishing date:

Info

Title:
Cloning and Expression Analysis of an Auxin Response Factor Gene in Cerasus pseudocerasus ‘Manaohong’
Author(s):
LI Xiaorong; HOU Qiandong; JIANG Yuwen; TIAN Tian; QIAO Guang
(Institute of Agro-bioengineering，Guizhou University/Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region，Ministry of Education，Guiyang，Guizhou 550025)
Keywords:
Cerasus pseudocerasus ‘Manaohong’; gene cloning; bioinformatics; expression analysis
PACS:
-
DOI:
10.11937/bfyy.20210600
Abstract:
CpARF7gene was cloned by RT-PCR technology from the root tissue of Cerasus pseudocerasus  ‘Manaohong’，and its bioinformatics analysis and expression analysis were carried out，in order to provide reference for further study on the regulation of the growth and development of C.pseudocerasus  ‘Manaohong’.The results showed that the open reading frame (ORF) of contained 3 498 bp and encoded 1 165 amino acid，three domains of B3 DNA/Auxin-resp family and AUX_IAA，and was the ARF transcription factor of domain integrity.The phylogenetic tree showed the closest relationship with Prunus avium homologous protein.  The results of real-tine PCR showed that the expression level of CpARF7[STBZ] gene in root，flower buds and ripening fruits tissue was significantly higher than those in other parts.Under 200 mg·L-1 IAA treatment，the expression level of in flower bud was induced，and the highest at 6 hours/24 hours.However，the expression level of in flower buds under 200 mg·L-1 ABA treatment was inhibited.These results indicated that CpARF7may be transcription factors related to the root system，flower bud，fruit growth and development of C.pseudocerasus ‘Manaohong’，and might be involved in the regulation of IAA/ABA hormone signal transduction in C.pseudocerasus ‘Manaohong’.

References:

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Last Update: 2021-12-23