|Table of Contents|

Cloning and Bioinformatics Analysis of [STBX]CcMYB8[STBZ] Gene From Cymbidium With Variegated Leaf

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年18
Page:
75-80
Research Field:
Publishing date:

Info

Title:
Cloning and Bioinformatics Analysis of [STBX]CcMYB8[STBZ] Gene From Cymbidium With Variegated Leaf
Author(s):
YAO Hongxu; LI Zhilin; ZHAO Yiran; CHENG Longjie; YANG Haonan; WANG Yuying
(School of Garden and Horticulture,Yunnan Agricultural University,Kunming,Yunnan 650201)
Keywords:
Cymbidium with variegated leaf; transcription factor; MYB8; sequence analysis; homology
PACS:
-
DOI:
10.11937/bfyy.20210492
Abstract:
The full length of [STBX]CcMYB8[STBZ] gene was cloned from the leaves of Cymbidium with variegated leaf (Cymbidium tracyanum var.‘huanghua’×C.iridioides D.Don.‘Yeyi’) by PCR,and its sequence was analyzed by bioinformatics,amino acid homology and genetic relationship,the function of the [STBX]CcMYB8[STBZ] gene were studied,in order to explain the regulation mechanism of R2R3-MYB subfamily transcription factors on leaf spot formation.The results showed that the [STBX]CcMYB8[STBZ] gene of Cymbidium with variegated leaf was an unstable hydrophilic protein with a full length of 657 bp,encodes 219 amino acids,a molecular weight of 24.974 kDa and two SANT conserved domains,and was highly homologous to Cymbidium sinense,with a similarity of 99.54%.The phylogenetic tree also showed that it was closely related to Cymbidium sinense,so the function of [STBX]CcMYB8[STBZ] gene is similar or the same as that of this species.

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Last Update: 2021-12-21