|Table of Contents|

Optimization of Culture Conditions for Laccase Production From Pleurotus ostreatus by Response Surface Methodology

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年20
Page:
132-138
Research Field:
Publishing date:

Info

Title:
Optimization of Culture Conditions for Laccase Production From Pleurotus ostreatus by Response Surface Methodology
Author(s):
LIU Xiaoxue1; WANG Qiang1; YANG Shili2; ZHENG Suyue1; GUO Jinying1
(1.Landscape and Ecological Engineering,Hebei University of Engineering College,Handan,Hebei 056038;2.Xingtai Vegetable Station,Xingtai,Hebei 054000)
Keywords:
white rot fungi; Pleurotus ostreatus; laccase; response surface methodology
PACS:
-
DOI:
10.11937/bfyy.20210469
Abstract:
Pleurotus ostreatus was used as the test material.On the base of single factor experiment,Box-Behnken design in response surface methodology were employed to establish mathematical model for optimization of liquid medium composition of Pleurotus ostreatus while the laccase activity was taken as the response in this study.In order to provide a theoretical basis and reference for the rapid degradation of lignin fiber.The results showed that glucose,yeast paste,Cu2+ and inoculation size were main composition for the laccase activity,the optimal composition to produce laccase from Pleurotus ostreatus was as follows glucose 52 g?L-1,yeast paste 9 g?L-1,Cu2+ 1.5 mmol?L-1,inoculation size 3%.Under the optimized conditions,the laccase activity was 359.12 U?g-1,which agreed closely with the theoretical result of 358.77 U?g-1 predicted by the developed model.Above results indicate that the response surface methodology was viable for optimizing medium composition in improving laccase activity.

References:

[1]刘宇,图力古尔.中国侧耳型真菌六个属的分子系统学研究[J].食用菌学报,2012(3):21-28.[2]史琦云,邵威平.八种食用菌营养成分的测定与分析[J].甘肃农业大学学报,2003(3):336-339.[3]艾良,朱启忠,李文静,等.白腐真菌F9产漆酶发酵条件的优化[J].北方园艺,2010(4):144-146.[4]刘岩,刘锐,苏新国,等.漆酶的研究进展及其应用[J].安徽农学通报,2016,22(13):25-27.[5]许颖,兰进.真菌漆酶研究进展[J].食用菌学报,2005,12(1):57-64.[6]BARI E，NAZARNEZHAD N，KAZEMI S M，et al.Comparison between degradation capabilities of the white rot fungi Pleurotus ostreatus and Trametes versicolor in beech wood[J].International Biodeterioration & Biodegradation,2015,104:231-237.[7]艾柳英,柯萍萍,赖春芬,等.两种食用菌漆酶基因克隆及表达分析[J].基因组学与应用生物学,2017，36(10):4197-4205.[8]卓睿,马富英,周帅,等.糙皮侧耳生长发育过程中漆酶基因家族的表达研究[J].菌物学报,2015,34(4):712-716.[9]王宁宁.血红栓菌发酵产漆酶的条件及酶学特性的研究[D].邯郸:河北工程大学,2018.[10]杨雪薇,张晓昱,龚青,等.铜离子调控木质纤维素降解和糙皮侧耳形态发育的研究[J].菌物学报，2015,34(4):647-652.[11]祝嫦巍,鲍广稳,黄顺.平菇漆酶对不同重金属胁迫的响应[J].环境科学研究,2015,28(10):1631-1637.[12]李志超,杨姗姗.食药用菌生产与消费指南[J].北京:中国农业出版社,1997.[13]李伟平.碳氮营养对秀珍菇生长发育及胞外酶活性的影响[D].保定:河北农业大学,2007.[14]尚洁,吴秋霞,练小龙,等.碳源和氮源对木蹄层孔菌产漆酶的影响及酶学性质研究[J].中国生物工程杂志，2013,33(11):32-37.[15]LEV1N L，FORCHIASSIN F，RAMOS A M.Copper induction of lignin-modifying enzymes in the white-rot fungus Trametes trogii[J].Mycologia，2002,94(3):377-383.

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Last Update: 2022-02-10