|Table of Contents|

Effects of pH on Physiological Characters of Agapanthus praecox Cell Proliferation in vitro

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年15
Page:
73-80
Research Field:
Publishing date:

Info

Title:
Effects of pH on Physiological Characters of Agapanthus praecox Cell Proliferation in vitro
Author(s):
YUE Jianhua1; DONG Yan2; LU Youfa1; GUO Cong1; GONG Shoufu1
(1.College of Horticulture,Xinyang Agriculture and Forestry University,Xinyang,Henan 464100;2.College of Forestry,Xinyang Agriculture and Forestry University,Xinyang,Henan 464100)
Keywords:
pH; cell proliferation; plant hormone; sugar metabolism; stress
PACS:
-
DOI:
10.11937/bfyy.20210282
Abstract:
Pedicel originated callus (Ca) and embryogenic callus (EC) were used as materials,endogenous hormone metabolism,sugar metabolism,reactive oxygen species and protective enzymes with gradient pH treatment were detected.The effects of gradient medium pH (5.6,5.8,6.0 and 6.2) on Ca,EC cell proliferation was studied,in order to investigate the effects of medium pH on the physiological characteristics of cell proliferation in vitro and provide reference for regeneration system optimization in Agapanthus praecox.The results showed that the optimal pH in Ca and EC cell proliferation was 5.8.At the stage of Ca proliferation,pH affected cell proliferation by regulating GA4,maltose and fructose accumulation.Subsequently,GA4,maltose and fructosewere significantly positively correlated with cell mass diameter,with correlation coefficients of 0.955,0.999 and 0.900,respectively.At the stage of EC proliferation,the contents of binding IAA,GA4,total saccharides and ·OH were positively correlated with the EC cell mass diameter,with correlation coefficients of 0.985,0.920,0.931 and 0.911,respectively.pH effects cell proliferation in vitro by regulating hormone metabolism,sugar metabolism and stress signal in Agapanthus praecox.

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Last Update: 2021-11-22