|Table of Contents|

Changes of Enzyme Activities Related to Glycolic Acid Synthesis During Fruit Development of Actinidia arguta

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年19
Page:
22-28
Research Field:
Publishing date:

Info

Title:
Changes of Enzyme Activities Related to Glycolic Acid Synthesis During Fruit Development of Actinidia arguta
Author(s):
ZHOU ZigengWANG ZiboJIN HualinAN JiaoPIAO Yilong
(Agricultural College,Yanbian University,Yanji,Jilin 133000)
Keywords:
Actinidia argutafruitsugar acid-related enzyme activity
PACS:
-
DOI:
10.11937/bfyy.20210191
Abstract:
Taking 7-year-old ‘Huanyou No.1’ ‘Kuilyu’ varieties and 5-year-old excellent line ‘Jiaohe 08-03’ as experimental materials,the changes of enzyme activities related to sugar and acid synthesis during fruit development were studied by spectrophotometer,in order to explore the mechanism of sugar and acid synthesis in soft jujube kiwifruit.The results showed that the accumulation of sucrose in fruit was mainly related to the activities of sucrose phosphate(SPS) and sucrose synthase (SS),but SPS contributed most,followed by SS,while acid invertase(AI) and neutral invertase(NI) had little effect on the accumulation of sucrose,in general,the sucrose content in fruit increased continuously.All the soft dates of the three lines were citric acid competitive fruit,phosphoenolpyruvate carboxylase(PEPC),citrate synthase(CS),malate dehydrogenase(NAD-MDH)and cytoplasm aconitase(Cyt-ACO) ointly promoted the accumulation of citric acid,but the largest contribution to the CS.However,the mitochondrial aconitase(Mit-ACO) and NAD-isocitrate dehydrogenase(NAD-IDH) were not conducive to citric acid accumulation.

References:

[1]RICHARDSON A C,BOLDINGH H L,PETER A M,et al.Fruit development of the diploid kiwifruit,Actinidia chinensis‘Hort16A’[J].BMC Plant Biol,2011(11):182.[2]KIM J G,BEPPU K,KATAOKA I.Physical and compositional characteristics of ‘Mitsuko’ and local hardy kiwifruits in Japan[J].Hortic Environ Biotec,2012,53:1-8.[3]周元,傅虹飞.猕猴桃中的有机酸高效液相色谱法分析[J].食品研究与开发,2013,34(19):85-87.[4]王刚,王涛,潘德林,等.不同品种猕猴桃果实有机酸组分及含量分析[J].农学学报,2017,7(12):81-84.[5]安娇,刘铭,贾佳林,等.软枣猕猴桃果实发育过程中糖酸组分及含量的变化[J].东北农业科学,2020,45(5):88-91.[6]张慧琴,谢鸣,张琛,等.猕猴桃果实发育过程中淀粉积累差异及其糖代谢特性[J].中国农业科学,2014,47(17):3453-3464.[7]黄宏文.猕猴桃属分类、资源、驯化、栽培[M].北京:科学出版社出版,2013.[8]张永平,乔永旭,喻景权,等.园艺植物果实糖代谢的研究进展[J].中国农业科学,2008,41(4):1151-1157.[9]周先艳,朱春华,李进学,等.果实有机酸代谢研究进展[J].中国南方果树,2015,44(1):120-125,132.[10]张志良,翟伟菁.植物生理学实验指导[M].3版.北京:高等教育出版社,2003.[11]王刚,王涛,潘德林,等.不同品种猕猴桃果实有机酸组分及含量分析[J].农学学报,2017,7(12):81-84.[12]NIELSEN T H,SKIARBEK H C,KARLSEN P.Carbohydrate metabolism during fruit development in sweet pepper(Capsicum annuum) plants[J].Physiol Plant,1991,82:311-319.[13]HIRAI M,UENO I.Development of citrus fruits:Fruit development and enzymatic changes in juice vesicle tissue[J].Plant and Cell Physiology,1977,18(4):791-799.[14]周晨卉.毛花猕猴桃‘华特’采后果实品质及其代谢特性的研究[D].杭州:浙江工商大学,2015.[15]陈美霞,赵从凯,陈学森,等.杏果实发育过程中糖积累与蔗糖代谢相关酶的关系[J].果树学报,2009,26(3):320-324.[16]章英才,陈亚萍,景红霞,等.‘灵武长枣’果实糖积累与蔗糖代谢相关酶的关系[J].果树学报,2014,31(2):250-257.[17]YATIV M,HARARY I,WOLF S.Sucrose accumulation in watermelon fruits:Genetic variation and biochemical analysis[J].Journal of Plant Physiology,2010,167:589-596.[18]文涛,熊庆娥,曾伟光,等.脐橙果实发育过程中有机酸合成代谢酶活性的变化[J].园艺学报,2001(2):161-163.[19]BLANKE M M,LENZ F.Fruit photosynthensis[J].Plant Cell and Environment,1980(12):31-36.[20]NOTTONB A,BLANK M M.Phosphoenolpyruvate carboxylase in avocado fruit:Parification and poroperties[J].Phytochemistry,1993(33):1333-1337.[21]翁金洋,薛松,倪照君,等.梅和杏果实有机酸代谢差异研究[J].南京农业大学学报,2018,41(6):1009-1017.[22]龚荣高,吕秀兰,张光伦,等.脐橙果实发育过程中有机酸代谢相关酶的研究[J].四川农业大学学报,2006(4):402-404.[23]李雪梅.砂梨果实有机酸含量及代谢相关酶活性动态变化研究[D].武汉:华中农业大学,2008.[24]陈昕.基于比较蛋白质组学骏枣和酸枣果实大小及糖酸含量差异机制研究[D].杨凌:西北农林科技大学,2019.[25]SADKA A,DAHAN E,COHEN L,et al.Aconitase activity and expression during the development of lemon fruit[J].Physiologia Plantarum,2000,108(3):255-262.[26]BOGIN E,WALLANEE.Citrate synthase from lemon fruit[J].Mothods in Enzymology,1966(13):19-22.

Memo

Memo:
-
Last Update: 2021-12-23