|Table of Contents|

Establishment of High-efficiency Adventitious Bud Regeneration From Leaf Explants of Malus domestica var.‘Luli’

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年19
Page:
42-47
Research Field:
Publishing date:

Info

Title:
Establishment of High-efficiency Adventitious Bud Regeneration From Leaf Explants of Malus domestica var.‘Luli’
Author(s):
SUN Qingrong; GUAN Qiuzhu; LI Linguang; HE Ping; WANG Haibo; SUN Hongyan
(Shandong Institute of Pomology,Tai′an,Shandong 271000)
Keywords:
apple; in vitro leaf explants; adventitious buds; adventitious shoots; adventitious roots
PACS:
-
DOI:
10.11937/bfyy.20210059
Abstract:
New expanding leaves from in vitro shoot top of new varieties ‘Luli’ (Malus domestica var.‘Luli’) with early mature and good quality were selected as materials.The effects of plant growth regulators and carbon source on adventitious bud regeneration from leaf explants were examined.The results showed that carbon source D-sorbitol than sucrose markedly improved adventitious bud regeneration frequency and averaged bud number per leaf explant,independent of plant growth regulators.Under the same carbon source,both bud regeneration frequency and bud number per leaf explant showed TDZ was higher than BA,but didn’t reach significant difference between TDZ and BA.For in vitro rooting of adventitious shoots,basal medium 1/4MS than 1/2MS significantly improved rooting rate,exclusive of adding higher IBA concentration 1.0 mg?L-1.On basal medium 1/4MS,auxin IAA than IBA significantly improved rooting rate and root number per plantlet.On basal medium 1/2MS,both rooting rate and root number per plantlet showed no significant difference between IAA and IBA.But rooting process induced by IAA was faster than that by IBA.Roots induced by IAA were directly produced from stem cross without callus production.The highest rooting rate was 80.8%,and root number per plantlet was 4.2.

References:

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Last Update: 2022-01-04