|Table of Contents|

Allelotoxic Effect of Aqueous Extract Solution From Pogostemon cablin

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年16
Page:
108-115
Research Field:
Publishing date:

Info

Title:
Allelotoxic Effect of Aqueous Extract Solution From Pogostemon cablin
Author(s):
LU Hong1; LI Ming1; 2; LI Longming1
(1.College of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Guangzhou,Guangdong 510006;2.Key Laboratory of Lingnan Crude Drugs Production and Development,National Administration of Traditional Chinese Medicine,Guangzhou,Guangdong 510006)
Keywords:
Pogostemon cablin; water extract liguid; phenolic acids; allelotoxic substances; continuous cropping obstacle
PACS:
-
DOI:
10.11937/bfyy.20210042
Abstract:
Taking the tissue culture acclimated plantlet of Pogostemon cablin as test material.The effects of water extracts from roots,stems and leaves of Pogostemon cablin on root activity,total chlorophyll content,malondialdehyde content,peroxidase (POD) activity,catalase (CAT) activity and superoxide dismutase (SOD) activity of Pogostemon cablin seedlings were studied by pot experiment with tissue culture domesticated seedlings.In addition,high performance liquid chromatography(HPLC) was used to detect the contents of protocatechuic acid,chlorogenic acid,vanillin acid,caffeic acid,eugenic acid,ferulic acid,benzoic acid and cinnamic acid in water extracts from Pogostemon cablin roots,stems and leaves,as well as the allelosensitive effects of vanillin acid,eugenic acid,ferulic acid,benzoic acid and cinnamic acid on Pogostemon cablin seedlings.In order to provide a reference basis for the mechanism and causes of continuous cropping disorders of Pogostemon cablin.The results showed that water extracts from roots,stems and leaves had allelopathic and concentration effects on Pogostemon cablin seedlings,and the allelopathic and self-toxic effects of water extracts from leaves were the most significant,and the types and contents of phenolic acids were higher than those from roots and stems.The content of phenolic acid in the aqueous extract of leaves was as follows,benzoic acid>protocatechuic acid>caffeic acid>ferulic acid>eugenic acid>vanillin acid>chlorogenic acid>cinnamic acid.The five phenolic acids had different inhibitory effects on plant height,root length,root number,fresh weight,leaf area,root activity and total chlorophyll content of Pogostemon cablin,and increased POD,CAT,SOD activities and MDA content of leaves,all of which had concentration effects.The allelopathic effects of the five phenolic acids on the Pogostemon cablin seedlings were compared as follows cinnamic acid>benzoic acid>ferulic acid>syringic acid>vanillic acid.The continuous dysfunction of Pogostemon cablin might be closely related to the water-soluble phenolic acid self-toxic substances in its organs.The phenolic acid substances such as cinnamic acid and benzoic acid might be the main allelopathic self-toxic substances.

References:

[1]国家药典委员会.中国药典Ⅰ部[S].北京:中国医药科技出版社,2015.[2]周芳,曹国璠,李金玲,等.药用植物连作障碍机制及其缓解措施研究进展[J].山地农业生物学报,2019,38(3):67-72.[3]张重义,林文雄.药用植物的化感自毒作用与连作障碍[J].中国生态农业学报,2009,17(1):189-196.[4]胡帅珂,高岩,王莘.水稻残茬自毒作用研究[J].现代农业科技,2012(5):51-52．[5]韩丽梅,王树起,鞠会艳,等.大豆根茬腐解产物的鉴定及化感作用的初步研究[J].生态学报,2000,20(5):771-778．[6]沙红,高燕,李雪源.棉花植株水提取液对种子萌发和幼苗生长的自毒作用[J].新疆农业科学,2012,49(5):826-830．[7]黄玉茜，韩立思，杨劲峰,等.花生植株和土壤水浸液自毒作用研究及土壤中自毒物质检测[J].生态学报，2012(19):6023-6032.[8]杨从军.番茄茎叶水提取液对7种蔬菜的化感作用[J].青岛农业大学学报(自然科学版),2019,36(2):91-94,111.[9]张文明,邱慧珍,张春红,等.连作马铃薯不同生育期根系分泌物的成分检测及其自毒效应[J].中国生态农业学报,2015,23(2):215-224．[10]王田涛,王琦,王惠珍,等.当归自毒作用和其对不同作物的化感效应[J].草地学报,2012,20(6):1132-1138．[11]马红梅,赵培芳.灵芝连作障碍下的自毒作用[J].北方园艺,2016(6):133-136．[12]冯静,周冰谦,刘谦,等.药用植物根际微生物与酚酸类自毒物质致害机制及作用关系研究[J].中华中医药杂志,2020,35(8):4049-4052.[13]BOOKER F L,BLUM U,FISCUS E L.Short-term effects of ferulic acid on ion uptake and water relations in cucumber seedlings[J].Journal of Experimental Botany,1992,43(5):649-655.[14]唐堃,李明,董闪,等.广藿香根际土壤水浸液对其扦插苗的化感自毒作用[J].中药材,2014,37(6):935-939.[15]唐堃,李明,赵盼,等.广藿香连作土壤对其扦插苗扦插生根的化感作用[J].广东农业科学,2014,41(6):85-88,237.[16]李玲梅.利用组织培养技术研究广藿香的化感自毒作用[D].广州:广东药学院,2012.[17]宫李辉,高振宇,马伯军,等.水稻粒形遗传的研究进展[J].植物学报,2011,46(6):597-605.[18]吴元华,浦选昌,杨昌元,等.黔东南禾稻类型分类及叶龄栽培模式研究[J].贵州农业科学,2010,38(1):49-52.[19]张志良,瞿伟菁.植物生理学实验指导[M].北京:高等教育出版社,2003.[20]李合生.植物生理生化试验原理和技术[M].北京:高等教育出版社,2009.[21]李玲,李娘辉,蒋素梅,等.植物生理学模块实验指导[M].北京:科学出版社,2009.[22]王园园,董淑琦,王宏富,等.谷子根系水浸提液的化感自毒效应研究[J].山西农业大学学报(自然科学版),2019,39(2):7-13.[23]黄钰芳,张恩和,张新慧,等.兰州百合根及鳞茎水浸液自毒作用的研究[J].草业学报,2017,26(8):93-103.[24]段修冉,李明,李龙明,等.广藿香不同茬口土壤对其幼苗农艺性状和百秋李醇含量的影响[J].中药材,2017,40(11):2516-2519.[25]唐堃,李明,董闪,等.广藿香根际土壤水浸液对其扦插苗的化感自毒作用[J].中药材,2014,37(6):935-939.[26]唐堃,李明,赵盼,等.广藿香连作土壤对其扦插苗扦插生根的化感作用[J].广东农业科学,2014,41(6):85-88,237.[27]孔垂华,胡飞.植物化感(相生相克)作用及其应用[M].北京:中国农业出版社,2001.[28]吕丰娟,肖运萍,汪瑞清,等.连作对不同抗性芝麻根系分泌物中酚酸类物质含量的影响及其化感作用[J].江西农业学报,2020,32(4):1-8.[29]李敏,闫兴富,马丽,等.酚酸类化感自毒物质对枸杞种子萌发的抑制作用[J].生态学报,2020,40(6):2072-2079.[30]汤绍虎,罗充.植物生物学教程[M].重庆:西南师范大学出版社,2012.

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Last Update: 2021-12-03