|Table of Contents|

Effects of Overexpression of [STBX]MsRCI2A,MsRCI2B and MsRCI2C[STBZ] Genes on Cold Tolerance of Alfalfa

《北方园艺》[ISSN:1001-0009/CN:23-1247/S]

Issue:
2021年18
Page:
67-74
Research Field:
Publishing date:

Info

Title:
Effects of Overexpression of [STBX]MsRCI2A,MsRCI2B and MsRCI2C[STBZ] Genes on Cold Tolerance of Alfalfa
Author(s):
ZHAN Lifeng; LI Chunxin; HE Kai; DONG Li; XU Huihui; CAI Hua
(College of Life Sciences,Northeast Agricultural University,Harbin,Heilongjiang 150030)
Keywords:
alfalfa; low temperature stress; [STBX]MsRCI2[STBZ] genes; transgenic alfalfa
PACS:
-
DOI:
10.11937/bfyy.20205515
Abstract:
Alfalfa was used as the test material.The expression differences of [STBX]RCI2 genes MsRCI2A/2B/2C[STBZ] derived from alfalfa were analyzed at 4 ℃,and then the genetic transformation of alfalfa was carried out by agrobacterium-mediated method.The effect of overexpression of [STBX]MsRCI2A/2B/2C[STBZ] gene on cold tolerance of alfalfa,in order to provide reference for cultivating cold tolerant alfalfa.The results showed that the [STBX]MsRCI2A/2B/2C[STBZ] genes were all induced by 4 ℃.The chlorophyll relative content,relative conductivity,and MDA content of the transgenic alfalfa changed significantly after 12 days of 4 ℃ treatment,indicating that their sensitivity to 4 ℃ was reduced.And after treatment at 4 ℃,the transgenic plants accumulated more proline and soluble sugar than WT plants,and the activities of antioxidant enzymes (SOD,POD and CAT) increased rapidly at 6 days of treatment at 4 ℃,and responded to cold stress more rapidly.In summary,the overexpression of [STBX]MsRCI2A/2B/2C[STBZ] genes improved the cold tolerance of alfalfa.

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Last Update: 2021-12-21